摘要
Rapid and simultaneous in situ detection of multi-components is extremely crucial for the real-time monitoring of nutrients in fruits.Herein,a facile and user-friendly poly(carboxybetaine methacrylate)-coated paper-based microfluidic device(pCBMA-μPAD)has been exploited to synchronously identify and semi-quantify vitamin C.glucose,sucro se and fructose in fruits.The pCBMA was successfully grafted from the surface of paper sensor using a co nvenient and robust method,which was confirmed by Fourier transform infrared spectroscopy(FT-IR)and X-ray photoelectron spectrometry(XPS).The superior hydrophilicity and ultra-low fouling of pCBMA endowed the pCBMA-μPAD with remarkably rapid response(3 min),high sensitivity,good linear relationship and low detection limit(LOD)(vitamin C:y=33.809+5.175 x,R^2=0.993,LOD=0.179 mmol/L;glucose:y=-0.113+30.0661 g(x),R^2=0.988,LOD=0.095 mmol/L;sucrose:y--5.334+34.858 lg(x),R^2=0.996,LOD=0.097 mmol/L;fructose:y=4.996+23.325 lg(x),R^2=0.994,LOD=0.140 mmol/L).Furthermore,satisfactory results were yielded in the detection of these nutrients in 9 fruits,which were much agreed well with those obtained by spectrophotometry.Such a portable and versatile pCBMA-μPAD will pro foundly shape the future of food analysis,especially for the assessment of food quality and nutrition in the process of agricultural production and marketing.
基金
financially supported by the National Natural Science Foundation of China(Nos.31701678,31801638)
the Key Project of Shanghai Agriculture Prosperity through Science and Technology(No.2019-02-08-00-15-F01147)
the Key Science and Technology Project of Henan(No.172102310586)
China Post-Doctoral Science Foundation(No.2018T110338)。
