摘要
目的探讨靶向敲除Yes激酶相关蛋白1(YAP1)对纳美芬治疗急性颅脑损伤疗效的影响。方法选取113只雄性SD大鼠随机分为假手术组9只、模型组31只、对照组36只和实验组37只。用自由落体法建立大鼠颅脑创伤模型。假手术组只开颅不撞击;模型组只进行颅脑创伤处理;实验组和对照组经颅脑创伤处理后,分别给予YAP1-shRNA重组慢病毒液(浓度1.0×10^8 TU·mL^-1,100μL,一次性腹腔注射)和shRNA慢病毒空载液(100μL,一次性腹腔注射),后再给予0.1 mg·kg^-1盐酸纳美芬溶液(腹腔注射,bid,共治疗2周)。治疗前,用蛋白质印迹法检测4组大鼠YAP1蛋白含量;治疗后,用流式细胞仪和免疫组化法分别检测4组大鼠神经细胞凋亡情况和内源性阿片肽[β-内啡肽(β-EP)和强啡肽(DynA1-13)]含量。结果治疗前,实验组、假手术组、对照组和模型组的YAP1蛋白水平分别为(0.13±0.04),(0.32±0.05),(0.83±0.16)和(0.85±0.14),其中实验组显著低于假手术组、对照组和模型组(均P<0.05),假手术组显著低于模型组和对照组,差异均有统计学意义(均P<0.05)。治疗后第14天,实验组、对照组和模型组的神经细胞凋亡率分别为(7.56±1.16)%,(11.78±2.17)%和(15.23±1.98)%,β-EP分别为(0.24±0.12),(0.15±0.08)和(0.31±0.17),DynA1-13分别为(0.37±0.15),(0.18±0.05)和(0.44±0.13),实验组的上述指标与对照组和模型组比较,差异均有统计学意义(均P<0.05)。结论 YAP1蛋白在急性颅脑损伤中高表达,靶向敲除其可明显提高纳美芬治疗急性颅脑损伤的效果。
Objective To explore the effect of targeted knockout Yes kinase associated protein 1(YAP1)on acute craniocerebral injury rats treated with nalmefene.Methods 113 male Sprague Dawley(SD)rats were randomly divided into sham operation group(n=9 cases),model group(n=31 cases),control group(n=36 cases)and experimental group(n=37 cases).The rat brain injury model was established by free falling method.The sham operation group only had craniotomy without impact;the model group only had craniocerebral trauma treatment;after craniocerebral trauma treatment,the experimental group and control group were given YAP1-shRNA recombinant lentivirus solution(concentration:1.0×10^8 TU·mL^-1,100μL,one-time intraperitoneal injection)and shRNA lentivirus empty carrier solution(100μL,one-time intraperitoneal injection)respectively,and then they were given 0.1 mg·kg^-1 namefen hydrochloride solution(intraperitoneal injection,twice a day for two weeks).Before treatment,the YAP1 protein of the four group were detected by protein blotting method;after treatment,the neuronal apoptosis and endogenous opioid peptide[beta-endorphin(β-EP)and dynorphin A1-13(DynA1-13)]of the four group were detected by flow cytometry and immunohistochemistry respectively.Results Before treatment,the levels of YAP1 protein in the experimental group,sham operation group,control group and model group were(0.13±0.04),(0.32±0.05),(0.83±0.16)and(0.85±0.14)respectively,the experimental group was significantly lower than the sham operation group,control group and model group(all P<0.05),and the sham operation group was significantly lower than the model group and control group,the differences had statistical significance(all P<0.05).The 14th day after treatment,the apoptotic rates of neurons in experimental group,control group and model group were(7.56±1.16)%,(11.78±2.17)%and(15.23±1.98)%,β-EP were(0.24±0.12),(0.15±0.08)and(0.31±0.17),DynA1-13 were(0.37±0.15),(0.18±0.05)and(0.44±0.13),and comparing with the control group and model group,the differences of the above indexes in the experimental group were statistically significant(all P<0.05).Conclusion YAP1 protein is highly expressed in acute craniocerebral injury,targeting knockout YAP1 can obviously improve the effect of nalmefene treating acute craniocerebral injury.
作者
林吴用
马宝林
涂献坤
LIN Wu-yong;MA Bao-lin;TU Xian-kun(Department of Neurosurgery,The First Affiliated Hospital of Xiamen University,Xiamen 361022,Fujian Province,China;Department of Neurosurgery,Fujian Medical University Union Hospital,Fuzhou 350000,Fujian Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2020年第11期1477-1480,共4页
The Chinese Journal of Clinical Pharmacology
基金
福建省卫生计生中青年骨干人才培养资助项目(2016-ZQN-28)。
关键词
纳美芬注射液
急性颅脑损
Yes激酶相关蛋白1
nalmefene injection
acute craniocerebral injury
Yes kinase associated protein 1
