摘要
为探究膜生物反应器(MBR)工艺对污水中常见的四环素抗性基因(tet A、tet W、tet X)和磺胺类抗性基因(sulⅠ、sulⅡ)的去除效果,评价回用水水质安全性,以某大型商业中心的污水为研究对象,采用MBR为核心的污水处理工艺,用q PCR(实时荧光定量聚合酶链式反应)技术分析A/O-MBRO3/AC组合工艺对ARGs的削减及去除机理。结果表明,在进水水质波动大、经常出现高污染高抗生素抗性基因丰度的情况,A/O-MBR-O3/AC组合工艺对水中各类ARGs的去除达2~3个数量级,使出水ARGs丰度稳定在10^-0.71~10^1.93copies/m L,低于城市再生水厂的三级出水,且常规出水指标符合回用水相关标准,具有良好的回用安全性;根据ARGs与16S r DNA和sulⅠ1的显著正相关性,发现膜过滤对微生物的截留作用是去除ARGs的主要因素,臭氧活性炭工艺不能有效去除ARGs,且出现负去除现象,以MBR为核心的污水处理工艺可有效去除水中的ARGs,且16S r DNA和Ⅰ类整合子(sulⅠ1)的去除有助于ARGs的削减。
The paper is inclined to make an investigation of the removal effect or efficiency of the membrane bioreactor(MBR)process on the antibiotic resistance genes(ARGs)in the public building sewage and assess the safety of its reuse.For the said purpose,we have traced and examined its variation of 5 ARGs,that is,the tet A,tet W,tet X,sulⅠ,and sulⅡ-in the sewage from a large commercial center with A/O-MBR-O3/AC process combined.In addition,efforts have been made to detect the usefulness of the absolute abundance of ARGs through the real-time quantitative polymerase chain reaction(q PCR)of each treatment process.Subsequently,we have also analyzed the mutual relation between the 16 S r DNA,intⅠ1 and the ARGs by checking thoroughly the Pearson bivariate correlation to disclose the removal mechanism.The results of our examination prove that,the influent water quality of the commercial center tends to fluctuate unsteadily whereas the pollutant with ARGs is high from time to time simultaneously.Since the combined process can remove various types of ARGs in the sewage by 2 to 3 orders of magnitude,the effluent of ARGs remains stable at 10^-0.71-10^1.93 copies/m L,which turns out much lower than the tertiary water effluent of the urban reused water plants.However,its conventional effluent index can still meet the demands of the recycled water standards in nice recycling safety.For instance,the membrane filtration in the microorganism retention remains the chief factor for ARGs removal.The significant positive correlation between the 16 S r DNA,intⅠ1 and the ARGs helps to prove that the ARGs effluent section of the MBR process remains stable at 10-0.33-101.76 copies/m L while the biomass can also be made minimized.What is more,the ozone activated carbon process fails to remove ARGs effectively,which should be said negatively for the removal process.Thus,it can be said that the antibiotic resistant genes could be effectively removed by the MBR-based sewage treatment thanks to the contribution of the removal of 16 S r DNA and intⅠ1 to the reduction of ARGs,though the ozone activated carbon treatment fails to contribute to the ARGs removal.
作者
冯萃敏
庆杉
安鑫悦
王婷
陈佳琪
FENG Cui-min;QING Shan;AN Xin-yue;WANG Ting;CHEN Jia-qi(Key Laboratory of Urban Stormwater System and Water Environment,Ministry of Education,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;National Demonstration Center for Experimental Water Environment Education,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;Engineering Department of Logistics Services Group,Beijing Language and Culture University,Beijing 100083,China)
出处
《安全与环境学报》
CAS
CSCD
北大核心
2020年第2期683-689,共7页
Journal of Safety and Environment
基金
国家自然科学基金面上项目(51678026)
北京建筑大学研究生创新项目(PG2019044)。
