摘要
以双阳梅花鹿鹿鲜胎为原料,通过单因素实验和响应面法优化鹿鲜胎多肽酶解工艺;采用超滤法和凝胶过滤层析法对鹿鲜胎多肽进行分离纯化,同时对鹿鲜胎多肽抗氧化能力进行研究。结果表明:鹿鲜胎多肽最佳酶解工艺为:酶解时间5 h,酶解温度54.5℃,pH7.0,加酶量5100 U/g,水解度为34.97%;从鹿鲜胎多肽中分离纯化出3组分子量10 kDa以下的多肽组分:DFP1、DFP2、DFP3;抗氧化研究结果表明DFP1自由基清除能力最强,·OH自由基和O2^-自由基最高清除率分别为81.47%、79.43%。综上,通过响应面法优化的鹿鲜胎多肽酶解工艺方便可行,制备得到的多肽具有较强抗氧化活性,为双阳梅花鹿资源的充分开发利用提供理论基础。
In this work,the Shuangyang sika deer fresh foetus were used as raw materials to optimize the enzymatic hydorlysis conditions of fresh deer foetus polypeptides by single-factor experiment and response surface methodology.Ultrafiltration and gel filtration chromatography were used to separate and purify the polypeptide from fresh deer fetus.Then the antioxidant capacity of the deer fresh fetal poly was studied.The results showed that the optimal enzymatic hydrolysis process of fresh deer fetal polypeptide was as follows:The enzymatic hydrolysis temperature was 54.5℃,the pH was 7.0,the enzyme dosage was 5100 U/g,the degree of hydrolysis was 34.97%.There were 3 groups of polypeptide components were isolated and purified from the deer fresh fetal polypeptide components with molecular weights below 10 kDa which were named DFP1,DFP2 and DFP3.The results of antioxidant experiments showed that DFP1 had the strongest scavenging ability.The highest scavenging rates of·OH radical and O2^- radical were 81.47% and 79.43%,respectively.In summary,the enzymatic hydrolysis process of deer fresh fetal polypeptide optimized by response surface method was convenient and feasible.The prepared polypeptide has strong antioxidant activity,which provides a theoretical basis for the full development and utilization of Shuangyang sika deer sources.
作者
赵宇
王铮
高晓晨
孙尧
高冷
ZHAO Yu;WANG Zheng;GAO Xiao-chen;SUN Yao;GAO Leng(School of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130012,China;Changchun University of Traditional Medicine,Jilin Institute of Ginseng Science,Changchun 130117,China)
出处
《食品工业科技》
CAS
北大核心
2020年第13期195-201,共7页
Science and Technology of Food Industry
基金
吉林省中医药科技项目(2019050)。
关键词
鹿鲜胎多肽
响应面法
酶解
分离纯化
抗氧化
deer fresh fetal polypeptide
response surface method
enzymatic hydrolysis
gel filtration chromatography
antioxidant
