摘要
NBS-LRR类基因家族是植物抗病R基因(Resistance gene)数量最多的一类,具有NBS(Nucleotide-binding site)和LRR(Leucine-leucine-repeat)结构域。甘薯(Ipomoea batatas)栽培种基因组已完成测序,但尚未注释,本研究对甘薯基因组序列进行外显子预测,得到甘薯染色体组全基因组蛋白序列,在此基础上进一步对NBS-LRR家族基因鉴定和分析表明,甘薯基因组中含有379个NBS-LRR家族基因,占全基因组基因总数的0.212%,其中N型亚家族120个,NL型103个,CNL型133个,TNL型22个,PN型1个。所有染色体上均有NBS-LRR家族基因分布,但数量明显不同,其中有60.9%的NBS-LRR基因序列呈簇状分布。NBS-LRR基因序列有15个保守结构域,在N端较为保守。研究结果为甘薯进一步开展NBS-LRR家族基因的功能研究和抗性育种提供了参考。
The NBS-LRR gene families possess the most abundant resistance genes in plants.Members of the NBS-LRR gene families contain nucleotide-binding site(NBS)and leucine-leucine repeat(LRR)domains.The genome of sweet potato(Ipomoea batatas)cultivar has been sequenced but the genes have not been annotated yet.In this study,we predicted the exons of sweet potato genome and obtained the proteins sequences,which were then used to identify and analyze genes of NBS-LRR family.There were 379 genes within NBS-LRR family,amounting to 0.212%of the total genes of sweet potato.The number of the N type,NL type,CNL type,TNL type and PN type was 120,103,133,22,and 1,respectively.All of the chromosomes had NBS-LRR family genes but varied in number and 60.9%of them were clustered.NBS-LRR genes included 15 conservative domains and the genes were conservative within N terminal domain.The results provide references for further studies on the function of NBS-LRR family genes and resistance breeding of sweet potato.
作者
黄小芳
毕楚韵
石媛媛
胡韵卓
周丽香
梁才晓
黄碧芳
许明
林世强
陈选阳
HUANG Xiao-Fang;BI Chu-Yun;SHI Yuan-Yuan;HU Yun-Zhuo;ZHOU Li-Xiang;LIANG Cai-Xiao;HUANG Bi-Fang;XU Ming;LIN Shi-Qiang;CHEN Xuan-Yang(Key Laboratory of Crop Biotechnology,Fujian Agriculture and Forestry University,Fujian Province Universities,Fuzhou 350002,Fujian,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Key Laboratory of Genetics,Breeding and Multiple Application of Crops,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)
出处
《作物学报》
CAS
CSCD
北大核心
2020年第8期1195-1207,共13页
Acta Agronomica Sinica
基金
福建省科技重大专项子专题(2017NZ0002-2)资助。
