摘要
目的探究免疫组织化学法检测抑癌基因PTEN、p27对子宫内膜癌(EC)及癌前病变的诊断价值。方法采用免疫组织化学法对20例正常子宫内膜、35例无非典型性子宫内膜增生、20例子宫内膜不典型增生和60例子宫内膜癌组织中抑癌基因PTEN及p27的表达进行检测,并分析其与EC组织病理分级、临床分期、肌层浸润深度、淋巴结转移及组织学病理类型的关系。分析子宫内膜不典型增生及EC组织中PTEN及p27蛋白表达的相关性。结果 PTEN及p27在正常子宫内膜、无非典型性内膜增生、内膜不典型增生及EC组织中阳性表达率逐渐下降(P<0. 05)。PTEN及p27的表达随着EC病理分级升高而显著降低(P<0. 05);内膜样癌组织中PTEN及p27阳性率明显低于非内膜样癌组织(P<0. 05);而PTEN及p27的表达与EC病理分期、基层浸润深度及淋巴结转移无关(P>0. 05)。子宫内膜不典型增生及EC组织中PTEN及p27蛋白表达正相关(r=0. 599,0. 809,P均<0. 05)。结论抑癌基因PTEN、p27表达缺失是子宫内膜癌发生的早期事件,可作为早期诊断子宫内膜癌及癌前病变有效标记物。
Objective To explore the diagnostic value of immunohistochemical detection of tumor suppressor genes PTEN and p27 in endometrial cancer(EC)and precancerous lesions.Methods Immunohistochemistry was used to detect the expression of tumor suppressor genes PTEN and p27 in 20 cases of normal endometrium,35 cases of endometrial hyperplasia,20 cases of endometrial atypical hyperplasia,and 60 cases of endometrial cancer.The relationship between EC histopathological grade,clinical stage,myometrial invasion depth,lymph node metastasis and histopathological type was analyzed.The correlation between endometrial dysplasia and PTEN and p27 protein expression was analyzed in EC tissues.Results The positive expression rates of PTEN and p27 in normal endometrium,endometrial hyperplasia,atypical hyperplasia of endometrium and EC tissues gradually decreased(P<0.05).The expression of PTEN and p27 decreased significantly with the increase of EC pathological grade(P<0.05);the positive rates of PTEN and p27 in endometrial carcinoma tissues were significantly lower than those in non-endometrium carcinoma tissues(P<0.05);the expression of p27 was not related to the pathological stage of EC,the depth of basal infiltration and lymph node metastasis(P>0.05).There was a positive correlation between endometrial dysplasia and PTEN and p27 protein expression in EC tissues(r=0.599,0.809,P<0.05).Conclusions Loss of expression of tumor suppressor genes PTEN and p27 is an early event of endometrial cancer and can be used as an effective marker for early diagnosis of endometrial cancer and precancerous lesions.
作者
焦艳
陆春燕
才秋敏
贺红梅
赵长燕
JIAO Yan;LU Chun-yan;CAI Qiu-min;HE Hong-mei;ZHAO Chang-yan(Department of Pathology,Qinhuangdao Maternal and Child Health Hospital,Qinhuangdao,Hebei 066000,China)
出处
《诊断病理学杂志》
2020年第5期331-335,共5页
Chinese Journal of Diagnostic Pathology
