摘要
为探究硝酸镧对乙醇致急性肝损伤小鼠的保护作用,本试验将84只雄性ICR小鼠按体重随机分为空白组、模型组以及硝酸镧A、B、C、D、E剂量组(0.02、0.1、0.2、1.0、2.0 mg/kg),每组12只。除空白组外,其余6组灌胃给药30 d后一次性经口灌胃50%的乙醇,建立急性肝损伤小鼠模型,测定小鼠体重、食物利用率、肝脏系数,并检测血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的活力,血清和肝组织中总超氧化物歧化酶(T-SOD)活力、谷胱甘肽(GSH)及丙二醛(MDA)的含量。研究结果表明:硝酸镧能显著降低小鼠血清ALT和AST的活力(P<0.05),其中硝酸镧C、E组血清ALT分别降低23.58、21.07 U/L,C、D、E组血清AST分别降低52.56、43.83、28.54 U/L。硝酸镧能显著提高小鼠血清和肝脏中抗氧化酶T-SOD的活力和抗氧化物质GSH的含量(P<0.05),硝酸镧B、C、D、E组血清中T-SOD活力分别增加27.53、38.74、48.01、53.96 U/mL,C、D、E组肝脏T-SOD活力分别增加15.87、18.6、17.78 U/mg prot,硝酸镧D、E组血清GSH分别增加5.94、7.86μmol/L,E组肝脏GSH增加0.83μmol/g prot。硝酸镧能显著降低脂质氧化产物MDA的含量(P<0.05),硝酸镧D、E组血清MDA分别降低4.13、4.41 nmol/mL,E组肝脏MDA降低0.64 nmol/mg prot。硝酸镧具有一定的抗氧化作用,能够改善乙醇所致小鼠的急性肝损伤,最佳保护作用剂量为1.0 mg/kg和2.0 mg/kg。
The objective was to investigate the protective effect of lanthanum nitrate on acute ethanol-induced liver injury in mice.Eighty-four male ICR mice were randomly divided into control group,model group and lanthanum nitrate A,B,C,D,E dose groups(0.02,0.1,0.2,1.0,2.0 mg/kg),12 mice in each group.Except for the control group,the other six groups were given 50%ethanol orally after 30-day gavage in order to establish an acute liver injury model.Then the body weight,food utilization rate and liver coefficients of mice were measured,and the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were measured,the activity of total superoxide dismutase(T-SOD),the content of glutathione(GSH)and malondialdehyde(MDA)in serum and livers were measured.The results showed that lanthanum nitrate significantly decreased the activities of ALT and AST in serum(P<0.05),ALT in groups C and E decreased 23.58 U/L and 21.07 U/L respectively,AST in groups C,D and E decreased 52.56,43.83 U/L and 28.54 U/L respectively;Lanthanum nitrate increased the activity of T-SOD and the content of GSH in serum and livers(P<0.05),the activity of T-SOD in the serum of groups B,C,D and E increased 27.53,38.74,48.01,53.96 U/mL respectively,the activity of T-SOD in the liver of group C,D and E increased by 15.87,18.6,17.78 U/mg prot respectively,the serum GSH in groups D and E increased by 5.94,7.86μmol/L respectively,and the liver GSH in group E increased by 0.83μmol/gprot;and significantly reduced the content of MDA in serum and livers(P<0.05),the serum MDA in groups D and E decreased 4.13,4.41 nmol/mL respectively,and the liver MDA in group E decreased 0.64 nmol/mg prot.Lanthanum nitrate has a protective effect on acute ethanol-induced liver injury in mice,and the optimal dosages of protection were 1.0 mg/kg and 2.0 mg/kg.
作者
李睿珺
周雅琳
刘伟
李雍
秦勇
于兰兰
陈宇涵
许雅君
LI Ruijun;ZHOU Yalin;LIU Wei;LI Yong;QIN Yong;YU Lanlan;CHEN Yuhan;XU Yajun(Department of Nutrition and Food Hygiene,School of Public Health,Peking University,Beijing 100191,China;Toxicological Research and Risk Assessment for Food Safety,Beijing 100191,China)
出处
《中国饲料》
北大核心
2020年第13期29-34,39,共7页
China Feed
基金
国家重点研发计划课题(2017YFC1600203)。
关键词
硝酸镧
稀土
急性乙醇致肝损伤
氧化应激
抗氧化
lanthanum nitrate
rare earth
acute ethanol-induced liver injury
oxidative stress
antioxidant effect
