摘要
【目的】为了进一步挖掘海洋贝类潜在的降血糖功能。【方法】选用华贵栉孔扇贝闭壳肌为原料,以抑制α-葡萄糖苷酶活性为评价指标,采用正交试验法和响应面分析法优化α-葡萄糖苷酶抑制肽的制备工艺。【结果】华贵栉孔扇贝(Chlamys nobilis)、马氏珠母贝(Pinctada fucata)和栉江珧(Atrina pectinate)等三种海洋贝类的闭壳肌酶解产物均对a-葡萄糖苷酶、a-淀粉酶和脂肪酶具有抑制活性;正交试验和响应面综合分析结果表明,华贵栉孔扇贝闭壳肌制备a-葡萄糖苷酶抑制肽的最佳工艺:复合蛋白酶酶添加量5500 U/g,酶解温度57℃,酶解pH=7,酶解时间4 h,其酶解液中小肽含量21.64 mg/mL,α-葡萄糖苷酶的抑制率达到31.53%。【结论】利用响应面法可优化制备α-葡萄糖苷酶抑制肽的工艺。
【Objective】In order to further explore the potential antihyperglycemic substances from marine shellfish.【Method】Based onα-glucosidase inhibition rate,orthogonal design and response surface analysis method were used for optimization the preparation process ofα-glucosidase inhibitory peptides from adductor muscle of Chlamys nobilis.【Result】All three adductor muscle hydrolysates,from Chlamys nobilis,Pinctada fucata and Atrina pectinate,possessed inhibitory activity againstα-glucosidase,α-amylase and lipase.The optimum technology conditions for preparation ofα-glucosidase inhibitory peptides were as follows:enzyme dosage(complex protease)of 5500 U/g,temperature of 57℃,pH=7,and hydrolysis time of 4 hours.Under this condition,the hydrolysate was with an oligopeptides concentration of 21.64 mg/mL and theα-glucosidase inhibition rate was 31.53%.【Conclusion】Response surface methodology could be used for optimization the preparation ofα-glucosidase inhibitory peptides from the adductor muscle of Chlamys nobilis.
作者
林海生
廖津
章超桦
秦小明
曹文红
高加龙
罗凯耀
LIN Hai-sheng;LIAO Jin;ZHANG Chao-hua;QIN Xiao-ming;CAO Wen-hong;GAO Jia-long;LUO Kai-yao(College of Food Science and Technology,Guangdong Ocean University,National Research and Development Branch Center for Shellfish Processing(Zhanjiang),Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;Shenzhen Institute of Guangdong Ocean University,Shenzhen 518108,China;Collaborative Innovation Center of Seafood Deep Processing(Dalian Polytechnic University),Dalian 116034,China)
出处
《广东海洋大学学报》
CAS
2020年第5期72-79,共8页
Journal of Guangdong Ocean University
基金
广东海洋大学博士启动项目(R17082)
广东海洋大学大学生创新创业训练项目(CXXL2019160)
国家贝类产业技术体系建设专项(CARS-49)
广东普通高等学校水产品高值化加工与利用创新团队项目(GDOU2016030503)
广东海洋大学创新强校工程重大科研成果培育计划(GDOU2017052606)。
关键词
酶解
生物活性肽
α-葡萄糖苷酶抑制率
响应面法
enzymatic hydrolysis
bioactive peptide
α-glucosidase inhibition rate
response surface methodology
