摘要
目的建立栀子中绿原酸含量的测定方法。方法采用紫外分光光度法,以60%乙醇为空白,在波长329nm处测定绿原酸含量。结果绿原酸在(2.5-15)μg/mL范围内,线性关系良好(r=0.9993),回归方程y=0.0457x+0.0005,平均加样回收率为98.1%,RSD为2.7%。结论紫外分光光度法操作简便、准确、重复性好,可作为栀子中绿原酸含量测定的方法。
Objective:To establish a method for determining the content of chlorogenic acid in gardenia.Methods:Ultraviolet spectrophotometry was used with 60%ethanol as the blank to measure the chlorogenic acid content at a wavelength of 329 nm.Results:Chlorogenic acid had a good linear relationship(r=0.9993)in the range of 2.5 to15μg/mL.Regression equation was y=0.0457 x+0.0005 and the average sample recovery rate was 98.1%,and RSD was 2.7%.Conclusion:Ultraviolet spectrophotometry is simple and accurate with good reproducibility and can be used as a method for the determination of chlorogenic acid in gardenia.
作者
罗娜
王雪
黄丽佳
陈建雯
冯国华
郭亚东
邓亮
LUO Na;WANG Xue;HUANG Li-jia;CHEN Jian-wen;FENG Guo-hua;GUO Ya-dong;DENG Liang(School of Pharmacy,Kunming Medical University and Key Laboratory of Natural Medicine and Pharmacology of Yunnan Province,Kunming 650500,China;Honghe Vocational College of Health,Mengzi 661101,China;Biomedical Engineering Center,Kunming Medical University,Kunming 650500,China)
出处
《云南中医中药杂志》
2020年第6期67-69,共3页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
基金
2019年国家自然科学基金项目(81960652)
2018年云南省科技厅基金项目(2018FB150)。
关键词
栀子
绿原酸
紫外分光光度法
含量测定
gardenia
chlorogenic acid
ultraviolet spectrophotometry
content determination
