摘要
为了挖掘小麦穗长和穗宽性状相关QTL,并为人工合成小麦优异基因资源的开发利用奠定基础,以普通小麦品种西农389×人工合成小麦材料KU98的F7∶8 RIL群体为试验材料,基于小麦55K SNP芯片对F7∶8群体分型结果绘制的遗传连锁图谱及2018和2019年调查统计的F7群体表型数据,对小麦穗长和穗宽性状进行了QTL定位分析。结果表明,在1A、2D、3A、5A和7B染色体上检测到10个与穗长性状相关的QTL,在2D、4D、5A、6A和7D染色体上检测到10个与穗宽性状相关的QTL,其中与穗长相关的qSL-2D.1和与穗宽相关的qSW-4D.1、qSW-5A.1在两年中均被检测到,标记区间分别为AX-111939856~AX-111497351、AX-169335104~AX-110618708、AX-108792246~AX-111048027。qSW-5A.1连续两年均为主效QTL,且与qSL-5A.2在同一标记区间,推测该位点具有一因多效的遗传效应或连锁遗传效应。
Spike length and width are important spike traits of wheat,which are closely related to yield.In this study,the F7∶8 RIL population constructed by crossing the synthetic wheat KU98 with the common wheat Xinong 389 was used for mapping.F7 individuals were genotyped by wheat 55K SNP array and spike phenotypes were investigated and analyzed in 2018 and 2019.QTL mapping program was used to generate high-density linkage map and mapping analysis.Ten QTLs related to spike length were detected on chromosomes 1A,2D,3A,5A and 7B,and ten spike width-related QTLs were detected on chromosomes 2D,4D,5A,6A and 7D.Spike length-related locus qSL-2D.1 and spike width-related loci qSW-4D.1 and qSW-5A.1 were consistent in 2018 and 2019,whose location intervals were AX-111939856-AX-111497351,AX-169335104-AX-110618708 and AX-108792246-AX-111048027,respectively.The major QTL qSW-5A.1 was located in the same interval as qSL-5A.2 which is speculated as a multi-effect or interlocking genetic effect.This study laid a foundation for the exploration of QTLs related to spike length and width and for the excellent gene resources of synthetic wheat.
作者
水志杰
安沛沛
刘天相
吴洪启
刘乐
史雪
王中华
SHUI Zhijie;AN Peipei;LIU Tianxiang;WU Hongqi;LIU Le;SHI Xue;WANG Zhonghua(College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2020年第6期656-664,共9页
Journal of Triticeae Crops
基金
陕西省重点研发农业一般项目(2018NY-056)
陕西省自然科学基础研究计划-青年项目(2017JQ3033)。
