摘要
为明确浙江省景宁县上山头云锦杜鹃Rhododendron fortunei叶斑病Cercospora rhododendri病原菌的种类及其生物学特性,采取组织分离法获得菌株DJ-1,采用柯赫氏法则验证试验证明该病原菌为云锦杜鹃叶斑病致病菌,通过形态学和分子生物学技术鉴定该病原菌为芦笋拟茎点霉Phomopsis asparagi。对病原菌生物学特性的研究结果表明,不同培养条件对该病原菌的菌丝生长、分生孢子产生量均有显著影响(P<0.05);在马铃薯葡萄糖琼脂培养基中菌丝生长最为适宜,该病菌可以利用多种碳源和氮源,最佳碳源和氮源为甘露醇和甘氨酸;病菌分生孢子产生的最佳培养基为马铃薯葡萄糖琼脂和马铃薯蔗糖琼脂培养基,最佳碳源为葡萄糖和果糖,最佳氮源为甘氨酸;当pH为8.0时,病原菌菌丝及分生孢子产生量为最佳;光照12 h+黑暗12 h交替条件下菌丝及产孢量为最佳;病原菌菌丝致死温度为60℃,10 min,分生孢子致死温度为55℃,10 min。本研究为芦笋拟茎点霉在浙江省内引起云锦杜鹃叶斑病的首次报道。
In 2017 and 2018, Rhododendron fortunei leaves infected by leaf spot disease were collected in Jingning, Zhejiang province for pathogen research. 9 strains were obtained and DJ-1 was isolated by single spore isolation which was verified by card of Koch’s postulation disease test as pathogenic bacteria. It was idendified Phomopsis asparagi by morphology and molecular biology techniques. Experiments showed that different culture condition had different mycelium growth and conidium quantity. It demonstrated that the PDA was the best for mycelium growth, mannitol and glycine for carbon and nitrogen source. The best medium for conidium quantity was PDA and PSA medium with the best carbon and nitrogen source of glucose and fructose, and glycine. The optimal pH for mycelium growth and conidium quantity was 8 and that of light was 12 hours of illumination + 12 hours of dark. The experiment showed that the lethal temperature was 55℃ for 10 minutes for conidium, and 60℃ for 10 minutes for the mycelium.
作者
刘浩凯
张辉
陈玲芳
仇辉康
王宗琪
刘卫荣
李丽
路媛媛
LIU Hao-kai;ZHANG Hui;CHEN Ling-fang;QIU Hui-kang;WANG Zong-qi;LIU Wei-rong;LI Li;LU Yuan-yuan(Jingning Natural Resources and Planning Bureau of Zhenjiang,Jingning 323500,China;Jingning Ecological Forest Administration of Zhejiang,Jingning 323500,China;Shanxi Agricultural University College of Life Sciences,Taigu 030801,China)
出处
《浙江林业科技》
2020年第2期9-16,共8页
Journal of Zhejiang Forestry Science and Technology
基金
景宁畲族自治县科技计划项目(2016A23)。
关键词
浙江景宁
云锦杜鹃
叶斑病
芦笋拟茎点霉
生物学特征
Zhejiang Jingning
Rhododendron fortunei
leaf spot disease
Phomopsis asparagi
biological properties
