摘要
将11个来源的泛酸合成酶(Pantothenate synthetase,PS)基因进行克隆,并将其在Escherichia coli BL21(DE3)中表达,构建成功的菌株进行酶学性质研究并在发酵罐上进行边发酵边转化试验。结果表明,来源于短短芽孢杆菌(Brevibacillus brevis)的PS酶活性最高,为1562.3 U/mL,最适温度为30℃,最适pH为7.0,K cat为115.4 s-1。以D-泛解酸和β-丙氨酸为底物,将重组菌株在5 L发酵罐中边发酵边转化,反应进行46 h后D-泛酸产量可达92.2 g/L,转化率为93.7%,时空产率为48.5 g/(L·d)。
In this study,11 source PS enzyme genes were cloned and expressed in Escherichia coli BL21(DE3),and the successful strain was constructed for enzymatic property study with simultaneous fermentation and transformation experiment on the fermentation tank.The results showed that the activity of PS derived from Brevibacillus brevis was the highest(1562.3 U/mL),and the optimal temperature was 30℃,the optimal pH was 7.0,and the K cat was 115.4 s-1.The recombinant strain was fermented and transformed in a 5 L fermenter with D-pantothenic acid andβ-alanine as substrates.After the reaction for 46 h,the yield of D-pantothenic acid reached 92.2 g/L,the conversion rate reached 93.7%,and the spatiotemporal yield was 48.5 g/(L·d).
作者
马玉玉
宋伟
冯小娜
刘佳
钱园园
MA Yu-yu;SONG Wei;FENG Xiao-na;LIU Jia;QIAN Yuan-yuan(College of Food Engineering,Anhui Science and Technology University,Fengyang,Anhui 233100,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)
出处
《食品与机械》
北大核心
2020年第6期28-35,共8页
Food and Machinery
基金
国家轻工技术与工程一流学科自主课题(编号:LITE2018-20)
江苏省科技支撑计划社会发展项目(编号:BE2018623)。
关键词
D-泛酸
泛酸合成酶
短短芽孢杆菌
酶法
边发酵边转化
D-pantothenic acid
pantothenate synthetase
Brevibacillus brevis
enzymic method
fermentation and transformation
