摘要
为研究香柠檬精油对自闭症大鼠焦虑行为与认知能力的作用,本实验采用SD大鼠制备自闭症动物模型,GC-MS分析香柠檬精油的挥发性化学成分,对造模成功的大鼠嗅吸精油治疗,通过行为学实验和脑内神经递质变化来研究精油功效。研究发现中浓度香柠檬精油显著增加模型鼠在旷场实验中的运动总路程与中央区域探索时间,以及高架十字迷宫中探索开臂的次数百分比,在三箱社交实验和第二次Morris水迷宫中,嗅吸香柠檬精油的子代模型鼠社交与学习记忆能力有改善趋势,但未呈现出显著性差异。嗅吸中浓度香柠檬精油显著降低海马区与前额叶皮层中多巴胺、5-羟色胺含量,提高海马区多巴胺相对代谢率和前额叶皮层中5-羟色胺相对代谢率。嗅吸香柠檬精油可以减少模型鼠的焦虑行为,其作用可能与调节大鼠海马区与前额叶皮层中的5-羟色胺以及多巴胺的代谢有关。
To study the effects of bergamot oil on relieving anxiety and improving cognitive ability in autistic rats,a model of autism of SD rats were established.GC-MS method was used to analyze the volatile chemical constituents of the bergamot oil.The offspring of the model group were used in the essential oil function evaluation study.The offspring received the essential oil or control treatment by inhalational ways.The effects of bergamot oil odor were evaluated by behavioral experiments,the changes of neurotransmitters in different brain regions were also detected.The results showed that the bergamot essential oil could significantly increase the total distance and central area exploration time of the rats in open field tests,as well as the percentage of entries into the open arms in the elevated plus maze tests compared to the control treatment.In the three-chambered social test and Morris water maze test,the bergamot essential oil treated group showed an improvement in social and cognitive abilities,but did not show significant differences compared to the control treatment group.Compared to the control treatment,the bergamot essential oil treatment reduced the content of dopamine and 5-hydroxy tryptamine in the hippocampus and prefrontal cortex,and increased the relative metabolic rate of dopamine in the hippocampus and the relative metabolic rate of 5-hydroxy tryptamine in the prefrontal cortex.In a conclusion,bergamot oil showed anti-anxiety effects in the autism model.The effects might be related to the regulation of 5-hydroxy tryptamine and dopamine in hippocampus and prefrontal cortex.
作者
王舒婷
张楠
姚雷
WANG Shu-ting;ZHANG Nan;YAO Lei(School of Agriculture and Biology,Shanghai Jiao Tong University;School of Design,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第7期1124-1133,共10页
Natural Product Research and Development
基金
植物精油对自闭症谱系障碍的辅助治疗研究项目(18H2H22000127)。
