摘要
本研究在分析金针菇减数分裂重组热点时发现B交配型位点可能存在第三个亚位点,为此对同核菌株’6-3’和’6-21’的B交配型位点进行全基因组注释,获得8对同源信息素受体基因(pheromone receptor gene,pr)和3对同源信息素前体基因(pheromone precursor gene,pp),组成了2个结构完整、相距326kb的亚位点α和β;’6-21’菌株还发现1个’6-3’菌株不具有的pr,组成第3个亚位点γ,它与亚位点α和β没有连锁。’6-3’和’6-21’配对形成异核体后出菇,分离获得142个单孢菌株,其B交配型有8种基因型,通过亲和性试验得到证实,同时还验证了第3个亚位点γ具有与α、β相同的功能。
During analysis of the meiotic recombination hotspots,we speculated that there might be a third mat-B sublocus presenting in the genome of Flammulina filiformis.The genomes of the homokaryotic strains‘6-3’and‘6-21’were annotated,and 8 paired homologous pheromone receptor genes and 3 paired homologous pheromone precursor genes were found,which constituted two mat-B subloci(αandβ)those were 326kb apart.An extra pheromone precursor gene was found in the strain‘6-21’,which was absent in‘6-3’,constituting the third mat-B sublocusγunlinked withαandβ.Heterocaryon formed by mating‘6-3’with‘6-21’was cultivated for fruiting,and 142 single spore strains were isolated from the fruit-bodies.As a result,there were 8 genotypes of mat-B confirmed by compatibility tests.The third sublocus of mat-B was verified to have the same function asαandβloci.
作者
赵琛
徐伟南
李依宁
王朦
杨环
马新斌
江玉姬
谢宝贵
ZHAO Chen;XU Wei-Nan;LI Yi-Ning;WANG Meng;YANG Huan;MA Xin-Bin;JIANG Yu-Ji;XIE Bao-Gui(Mycological Research Center of Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第6期1090-1099,共10页
Mycosystema
基金
国家重点研发计划项目(2019YFC1710501)。
关键词
连锁
信息素前体
信息素受体
锁状联合
基因组重测序
linkage
pheromone precursor
pheromone receptor
clamp connection
genome resequencing
