摘要
试验旨在研究象草、玉米秸秆、稻草和甘蔗尾叶对水牛瘤胃体外发酵参数和菌群结构的影响。体外培养底物0.5 g,每组4个重复,体外模拟瘤胃发酵培养24 h,测定粗饲料降解特性、瘤胃发酵液的挥发性脂肪酸(VFA)和微生物结构。结果表明:玉米秸秆干物质降解率(DMD)显著高于象草、稻草和甘蔗尾叶(P<0.05)。象草、稻草和甘蔗尾叶3组总挥发性脂肪酸产量显著低于玉米秸秆组(P<0.05),玉米秸秆组乙酸产量显著高于象草和甘蔗尾叶组(P<0.05),玉米秸秆组丙酸产量显著高于象草和稻草组(P<0.05)。稻草组的木聚糖酶活性显著低于其他3组(P<0.05),象草组、玉米秸秆组和稻草组的淀粉酶活性显著高于甘蔗尾叶组(P<0.05)。各组间优势菌群皆为杆菌目(Bacteroidales)和梭菌目(Clostridiales)。由此可见,象草、玉米秸秆、稻草和甘蔗尾叶4组粗饲料具有相似体外发酵特性,其中玉米秸秆组综合降解效果最好。
The study aimed to evaluate effect of elephant grass,corn stalks,straw and sugarcane tail on fermentation degradation characteristics and microbial flora in vitro.The substrate was cultured in vitro with 0.5 g,four replicates in each group,and rumen fermentation in vitro for 24 h.The degradation characteristics of the roughage,the VFA of rumen fermentation and the microbial structure were broth determined.The results showed that the DMD of corn straw was significantly higher than that of elephant grass,straw and sugarcane tail(P<0.05).The total volatile fatty acid production of elephant grass,straw and sugarcane tail was significantly lower than corn stalk group(P<0.05).The yield of acetic acid in corn stalk group was significantly higher than that in elephant grass and the sugarcane tail group(P<0.05),and propionic acid in corn stalk group was significantly higher than that in elephant grass and the straw group(P<0.05).The xylanase activity in the straw group was significantly lower than the other three groups(P<0.05),amylase activity in the elephant grass group,corn stalk group and straw group was significantly higher than that in the sugarcane tail treatment group(P<0.05).The dominant bacteria in each group were Bacteroidales and Clostridiales.The results indicate that elephant grass,corn stalks,straw and sugarcane tail had similar in vitro fermentation characteristics,and the corn stalks group had the best comprehensive degradation effect.
出处
《饲料研究》
CAS
北大核心
2020年第6期6-11,共6页
Feed Research
基金
国家自然科学基金项目(项目编号:31560649)
国家重点研发计划项目(项目编号:2018YFD0501602、2016YFD0500507)
广西水产畜牧科技推广应用项目(项目编号:201528007)。
关键词
粗饲料
体外发酵
微生物多样性
roughage
in vitro fermentation
microbial diversity
