摘要
目的探讨维生素B 6修饰对四氧化三铁纳米酶(IONzymes)活性的影响以及对口腔变异链球菌(S.mutans)的抑制作用。方法通过溶剂热法合成IONzymes并进行维生素B 6修饰,观察经维生素B 6修饰的四氧化三铁纳米酶(VB 6-IONzymes)形貌的电镜表征,分析其催化活性的改变。结果与未经修饰的IONzymes相比,VB 6-IONzymes体积大幅减小,过氧化物酶活性显著增强。VB 6-IONzymes具有显著的抗口腔S.mutans的作用(P<0.001),且能显著促进1%过氧化氢(H 2O 2)对S.mutans形成生物膜的抑制作用(P<0.001)。讨论维生素B 6修饰不仅可以提高IONzymes的过氧化物酶与过氧化氢酶活性,还在抗口腔龋齿方面具有潜在的应用价值。
Objective To investigate the influence of vitamin B6 modification on enzymatic activity of ferric iron oxide nanozymes(IONzymes)and its inhibitory effect on oral Streptococcus mutans(S.mutans).Methods IONzymes was modified with vitamin B6 by a solvothermal reaction.The morphology of IONzymes with vitamin B6 modification(VB6-IONzymes)was observed by electron microscope,and change of its catalytic activity was analyzed.Results Compared with the unmodified IONzymes,the volume of VB6-IONzymes was greatly reduced and its peroxidase activity was significanly enhanced.Bacteriostatic experiment showed that VB6-IONzymes had a significant ability to inhibit S.mutans,and can significantly promote the inhibitory effect of 1%H2O2 on the S.mutans biofilm formation.Conclusion Vitamin B6 modification not only improves the enzymatic activity of IONzymes,but also shows potential application value in preventing dental caries.
作者
成荣
王萧
程璐
张唯
丁黎明
高利增
许卓斌
CHENG Rong;WANG Xiao;CHENG Lu;ZHANG Wei;DING Liming;GAO Lizeng;XU Zhuobin(Medical College,Yangzhou University,Yangzhou,Jiangsu,225001;Institute of Translational Medicine,Yangzhou University,Yangzhou,Jiangsu,225001)
出处
《实用临床医药杂志》
CAS
2020年第8期1-5,共5页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金资助项目(81901879)
江苏省大学生创新创业训练计划项目(201711117036Z)
江苏省大学生创新创业训练计划项目(201811117110E)
扬州大学大学生学术科技创新基金项目(X20190727)。
关键词
纳米酶
四氧化三铁
变异链球菌
催化
生物膜
nano-enzyme
ferric iron oxide
Streptococcus mutans
catalysis
biofilm
