摘要
目的:探讨沉默TRIM26表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭与迁移等生物学行为的影响及机制。方法:采用免疫组化和qRT-PCR法检测50例乳腺癌组织和相应癌旁正常组织中TRIM26蛋白与mRNA的表达。取对数生长期MCF-7细胞分为空白对照组(正常培养)、siRNA-NC组(转染阴性对照siRNA-NC)和siRNA-TRIM26组(转染siRNA-TRIM26),转染后分别通过CCK-8、细胞集落形成实验、Annexin V-FITC/PI染色、Transwell实验检测3组细胞的吸光度值、集落形成数目、细胞周期、凋亡率、侵袭与迁移能力,Western blot法检测p-PI3K、PI3K、p-AKT、AKT、p-mTOR和mTOR蛋白的表达。结果:乳腺癌组织中TRIM26蛋白与mRNA表达较癌旁正常组织中均升高(P<0.001)。与空白对照组和siRNA-NC组比较,siRNA-TRIM26组MCF-7细胞吸光度值降低,阻滞于G2/M期,细胞凋亡率升高,侵袭与迁移能力下降,且p-PI3K、p-AKT、p-mTOR蛋白表达下降(P<0.05)。结论:沉默TRIM26表达可抑制乳腺癌MCF-7细胞增殖、侵袭与迁移能力,并诱导细胞凋亡,其机制可能与抑制PI3K/AKT/mTOR信号通路有关。
Aim:To investigate the effects and mechanism of silencing TRIM26 expression on proliferation,apoptosis,invasion,migration and other biological behaviors of breast cancer cells MCF-7.Methods:Immunohistochemical staining and qRT-PCR were used to detect the expressions of TRIM26 protein and mRNA in 50 cases of breast cancer tissue and corresponding normal tissue adjacent to the cancer.MCF-7 cells were divided into blank control group(normal culture),siRNA-NC group(transfection of negative control siRNA-NC)and siRNA-TRIM26 group(transfection of siRNA-TRIM26).CCK-8,cell colony formation experiment,Annexin V-FITC/PI staining,Transwell experiment were used to detect the A value,number of colony formation,cell cycle distribution,apoptosis rate,invasion and migration ability of cells in each group.Western blot was used to detect the expressions of p-PI3K,PI3K,p-AKT,AKT,p-mTOR and mTOR protein.Results:The expressions of TRIM26 protein and mRNA in breast cancer tissue were significantly increased compared with normal tissue adjacent to cancer(P<0.001).Compared with the blank control group and the siRNA-NC group,the A value of MCF-7 cells in the siRNA-TRIM26 group decreased,the cells were blocked in the G2/M phase,and the expressions of p-PI3K,p-AKT,p-mTOR protein decreased(P<0.05).Conclusion:Silencing the expression of TRIM26 could inhibit the proliferation,invasion and migration of breast cancer cells MCF-7,induce apoptosis,and the mechanism may be related to the inhibition of PI3K/AKT/mTOR signaling pathway.
作者
张波
高峡
ZHANG Bo;GAO Xia(The First Department of Surgical Oncology, the Second People′s Hospital of Yichang(the Second People′s Hospital of Three Gorges University), Yichang, Hubei 443000)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2020年第4期516-520,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
湖北省自然科学基金项目(2018CFC031)。
