摘要
为了解云南省中缅边境地区牛口蹄疫免疫状况,评估疫情发生风险,对2016—2019年采自临沧市镇康、耿马和沧源3个县168个场点的入境牛和境内牛血清1688份,采用口蹄疫病毒O、A、Asia-1型C-ELISA抗体检测试剂盒与口蹄疫病毒非结构蛋白抗体单抗阻断ELISA试剂盒进行检测。结果显示:O、A、Asia-1型抗体阳性率分别为39.99%、39.22%、28.85%,非结构蛋白抗体阳性率为20.44%。与沧源县和耿马县相比,镇康县O、A、Asia-1型抗体阳性率和非结构蛋白抗体阳性率显著偏低(P<0.01)。2016—2019年,O、A型抗体阳性率总体呈显著上升趋势(P<0.05),Asia-1型呈下降趋势,非结构蛋白抗体呈现波浪状变化。境内牛O、A、Asia-1型抗体阳性率显著高于入境牛(P<0.05),而非结构蛋白抗体阳性率显著低于入境牛(P<0.01);水牛O、A、Asia-1型抗体阳性率和非结构蛋白抗体阳性率均高于黄牛。结果表明,云南省临沧市中缅边境地区入境牛口蹄疫免疫水平较低,且隐性带毒率较高,存在较高的病毒传入和疫情发生风险。提议在中缅边境地区持续开展牛口蹄疫病原学和血清学监测,提高边境地区易感动物免疫密度,严格监控牛的非法跨境移动。本研究为中缅边境地区牛口蹄疫防控提供了数据参考。
In order to know the status of immunization against foot-and-mouth disease(FMD)in cattle and buffalo in China-Myanmar border regions in Lincang City of Yunnan Province and to evaluate the risk of FMD outbreak,1688 serum samples were collected from imported and domestic cattle and buffalo in 168 farms located at 3 bordering counties including Zhenkang,Gengma and Cangyuan of Lincang during 2016 to 2019 for the detections of antibodies against FMDV O,A and Asia-1 by C-ELISA kit and antibodies against non-structure protein(NSP)by monoclonal antibody blocking ELISA.The results showed that the antibody positive rates of FMDV O,A and Asia-1 were 39.99%,39.22%and 28.85%respectively,and the positive rate of NSP antibody was 20.44%in tested animals.All of the positive rates were lower in Zhenkang County(P<0.01),as compared to those in Cangyuan and Gengma counties.The antibody positive rates of FMDV O and A trended to increase(P<0.05),that of Asia-1 type was going to decline,and that of NSP antibody wavily changed.In addition,the antibody positive rates of O,A and Asia-1 in domestic animals were higher than those in imported animals(P<0.05),while the NSP antibody positive rates was lower than that of imported cattle(P<0.01).The antibody positive rates of O,A and Asia-1 as well as NSP in buffalo were higher than those in cattle.It was concluded that the immunization level of FMD in the border regions was relatively low,and the latent virus carrying rate was high,indicating the higher risk of virus introduction and outbreak.It was suggested that etiological and serological surveillance of FMD should be continued in the border regions,the immune density of susceptible animals should be improved,also any illegal cross-border movement of cattle and buffalo should be strictly monitored.In conclusion,this study provides the data for references for prevention and control of FMD in China-Myanmar border regions.
作者
杨静竹
苗海生
陈云明
蔡高萍
谭东
李亚琴
黄晓雨
李乐
寇美玲
廖德芳
李华春
Yang Jingzhu;Miao Haisheng;Chen Yunming;Cai Gaoping;Tan Dong;Li Yaqin;Huang Xiaoyu;Li Le;Kou Meiling;Liao Defang;Li Huachun(Lincang Animal Disease Prevention and Control Center,Lincang,Yunnan 677000,China;Yunnan Academy of Animal Science and Veterinary,Kunming,Yunnan 650224,China)
出处
《中国动物检疫》
CAS
2020年第8期11-16,共6页
China Animal Health Inspection
基金
国家重点研发计划项目(2017YFC1200500)
农业国际交流与合作项目(SEACFMD)。
关键词
口蹄疫
中缅边境
血清学检测
非结构蛋白
FMD
China-Myanmar border
serological detection
non-structural protein
