摘要
目的对郑州市23例严重急性呼吸综合征冠状病毒2(SARS-CoV-2)核酸单靶标阳性标本进行跟踪检测分析,为实验室检测提供参考。方法按照《新型冠状病毒肺炎防控方案》采集新型冠状病毒肺炎(COVID-19)疑似患者的鼻拭子或咽拭子,提取SARS-CoV-2核酸并采用实时荧光逆转录聚合酶链反应(RTPCR)检测。对6例开放读码框1ab(ORF1ab)基因单靶标阳性和17例核壳蛋白(N)基因单靶标阳性患者进行重新采样检测。结果首检23例SARS-CoV-2核酸单靶标阳性的标本中有8例疑似病例在随后的重新采样检测中被确认为SARS-CoV-2核酸阳性,重新采样检测阳性率为34.78%。结论 COVID-19同种类型标本2次采样检测中均出现单靶标阳性的检测结果时,应该慎重对待,建议重新采样跟踪检测。
Objective To perform tracking detection from the first detection of 23 cases of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) nucleic acid single target positive samples in Zhengzhou,and to provide a reference for clinical laboratories.Methods According to the Pneumonia Prevention and Control Plan for Novel Coronavirus Infection,nasal swab and oropharyngeal swab samples from suspected corona virus disease 2019(COVID-19) patients were collected.The nucleic acid was extracted and detected by real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).Totally,6 open reading frame lab(ORF1ab) gene and 17 nucleocapsid protein(N) gene single target positive samples were detected.Results Totally,8 samples were positive in the tracking detection,and the positive conversion rate was 34.78%.Conclusions The samples detected single target positive for 2 times should be treated seriously and detected for tracking detection.
作者
牛卫东
许太彬
孙丽梅
安戈
史军
李羿
赵瑞臻
NIU Weidong;XU Taibin;SUN Limei;AN Yi;SHI Jun;LI Yi;ZHAO Ruizhen(Zhengzhou Center for Disease Control and Prevention,Zhengzhou 450007,Henan,China)
出处
《检验医学》
CAS
2020年第7期633-636,共4页
Laboratory Medicine
关键词
严重急性呼吸综合征冠状病毒2
新型冠状病毒肺炎
实时荧光逆转录聚合酶链反应
单靶标阳性
Severe acute respiratory syndrome coronavirus 2
Corona virus disease 2019
Real-time fluorescence reverse transcription polymerase chain reaction
Single target positivity
