摘要
为研究费菜黄酮对意大利青霉的抑菌效果并揭示其抑菌机制,本试验采用损伤接种法接种意大利青霉至柑橘,观察0、0.3、0.6、1.2 mg·mL^-1费菜黄酮对柑橘青霉病的控制效果,探究其对意大利青霉细胞膜通透性、细胞成分释放、菌丝表面结构、菌丝丙二醛(MDA)和过氧化氢(H2O2)含量等指标的影响。结果表明,费菜黄酮能够显著抑制菌丝生长,1.75 mg·mL^-1浓度下的抑制率为75.82%,7.00 mg·mL^-1浓度下的抑制率达到100%。经费菜黄酮处理后,意大利青霉细胞膜通透性显著增加,糖类等胞内物质释放至细胞外;扫描电镜显示菌丝体结构遭到破坏,菌丝体表面出现褶皱、塌陷;同时引起H2O2和MDA含量增加;柑橘果实的发病率和病斑直径减小。本研究结果为天然杀菌剂的研制以及抑菌机制的后续研究提供了一定的理论依据。
In order to study the inhibitory effect of flavonoids from Sedum aizoon L.on Penicillium italicum(P.italicum)and reveal the inhibitory mechanism.P.italicum was inoculated to citrus by injury inoculation method,the inhibition of flavoroids with the concentration of 0,0.3,0.6 and 1.2 mg·mL^-1 on blue mold were observed,and the cell membrane permeability,release of cellular components,hyphal surface structure observation,malondialdehyde content and hydrogen peroxide content were investigated.The results showed that the flavonoids from Sedum aizoon L.significantly inhibited the mycelial growth,and the inhibition rates at the concentration of 1.75 mg·mL^-1 and 7.00 mg·mL^-1 were 75.82%and 100%,respectively.After the treatment of flavonoids,the membrane permeability of P.italicum was significantly increased,and intracellular substances such as sugars were released outside the cell.Scanning electron microscopy(SEM)showed that the mycelium structure was destroyed,and the surface of the mycelium was wrinkled and collapsed.Meanwhile,flavonoids could cause the accumulation of hydrogen peroxide(H2O2)and increase the contents of malondialdehyde(MDA).The disease incidence and lesion diameter were also decreased compared with the control citrus fruits.This result would provide theoretical basis for development of new natural fungicides and further study of the antifungal mechanism.
作者
罗洁
王鸿飞
许凤
王靖
池宗豫
谢柯沁
吴承豪
邵兴锋
LUO Jie;WANG Hongfei;XU Feng;WANG Jing;CHI Zongyu;XIE Keqin;WU Chenghao;SHAO Xingfeng(College of Food and Pharmaceutical Science,Ningbo University,Ningbo,Zhejiang 315211)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第8期1737-1745,共9页
Journal of Nuclear Agricultural Sciences
基金
浙江省公益项目(LGN19C200012)
浙江省重点研发项目(2017C02021)
浙江省自然科学基金(LY16C200003)
宁波市自然科学基金(2015A610273)。
关键词
费菜黄酮
抑菌活性
柑橘
意大利青霉
细胞膜
flavonoids from Sedum aizoon L.
antifungal activity
citrus
Penicillium italicum
cell membrane
