摘要
为创制花生优良新种质,以花生品种珍珠红1号的干种子为材料,采用浸泡法开展甲基磺酸乙酯(EMS)诱变研究。结果表明,以半致死剂量为选择标准,适宜诱变条件为0.8%EMS处理10 h;筛选获得9个优良M5突变系,其中4个表现出产量高、品质优、综合性状优良等特点。上述4个M5突变系干荚果产量较对照(珍珠红1号)增加10.93%~26.78%,出仁率均高于对照;在品质方面,诱珍珠红1号2Ⅱ/19油酸含量为56.68%、油酸/亚油酸比值(O/L)为1.92,分别较对照提高8.44个百分点和0.57;诱珍珠红1号2Ⅲ/8亚油酸含量为39.05%,较对照提高3.28个百分点;诱珍珠红1号2Ⅲ/10粗脂肪含量为54.52%,较对照提高1.50个百分点;诱珍珠红1号3Ⅰ/8油酸含量为53.70%、O/L为1.71,分别较对照提高5.46个百分点和0.36。ISSR分子标记结果表明,9个优良M4突变系与珍珠红1号在分子水平上均存在明显差异,突变系之间在分子水平上也存在明显差异。本研究结果为花生遗传改良和功能基因研究提供了优良的新种质,为花生EMS诱变育种研究提供了参考。
In order to develop superior peanut germplasms, the effect of ethyl methane sulfonate(EMS) mutagenesis on dry seeds of Zhenzhuhong 1 was studied based on soaking method. The results showed that with semi-lethal rate as the selection criterion, the appropriate mutagenesis condition was 0.8% EMS treatment for 10 hours. Nine superior M5 mutants were developed, four mutants showed high yield, good quality and superior comprehensive characters. Compared with Zhenzhuhong 1(CK), their dry pod yields increased by 10.93% ~ 26.78%, and their shelling percentages were also higher. The oleic acid content of You Zhenzhuhong 1 2Ⅱ/19 was 56.68%, 8.44 percentage points more than CK;and the ratio of oleic acid to linoleic acid(O/L) was 1.92, 0.57 more than CK. The linoleic acid content of You Zhenzhuhong 1 2Ⅲ/8 was 39.05%, 3.28 percentage points more than CK. The crude fat content of You Zhenzhuhong 1 2Ⅲ/10 was 54.52%, 1.50 percentage points more than CK. The oleic acid content of You Zhenzhuhong 1 3Ⅰ/8 was 53.70%, 5.46 percentage points more than CK;and its O/L was 1.71, 0.36 more than CK. Analysis of inter-simple sequence repeat(ISSR) markers revealed that there were significant molecular differences between the nine superior M4 mutants and Zhenzhuhong 1, and there were also significant molecular differences among these mutants. This study has created superior germplasms for peanut genetic improvement and functional gene research, providing a reference for peanut breeding by EMS mutation.
作者
许燕
谢永平
郑楚群
陈育华
陈肇聪
李辉
XU Yan;XIE Yongping;ZHENG Chuqun;CHEN Yuhua;CHEN Zhaocong;LI Hui(Shantou Agricultural Science Research Institute,Shantou,Guangdong 515021)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第7期1369-1377,共9页
Journal of Nuclear Agricultural Sciences
基金
汕头市科技计划项目(2014-70)。
