摘要
目的通过研究证实蒙药蓝刺头防治绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)的作用。基于蒙医药"补暖补精-清镇赫依-固骨质壮骨"经典理论,通过防治PMOP提供实验依据,揭示此理论、治法效应,为蒙医药现代化提供思路。方法分组:6月龄SD系SPF级健康雌性未孕大鼠84只,体重(250±20) g,随机分为假手术组(Sham)、去卵巢骨质疏松模型组(OVX)、蒙药蓝刺头高剂量组(Echinops-H)、中剂量组(Echinops-M)、低剂量组(Echinops-L)、中药对照组淫羊藿组(HEP)、西药对照组辅酶Q10组(Co-Q10) 7组。造模:采用双侧卵巢切除法(去势)制备绝经后骨质疏松模型,阴道上皮角化实验、骨密度检测、子宫病理学检查验证造模成功。给药:术后90 d,模型组、假手术组给予生理盐水灌胃,其余各组予相应药物灌胃。标本采集:末次灌胃后行标本采集。处死大鼠剔除右侧完整股骨并标记,4%多聚甲醛溶液固定保存。指标检测:右侧股骨采用骨密度仪测定离体骨骨密度(bone mineral density,BMD)(股骨近端BMD、股骨总BMD)检测,行Micro-CT骨微结构形态计量学测定。结果去卵巢术后各组大鼠BMD下降明显,表现为松质骨、皮质骨骨量相继减少,皮质骨变薄,BMD下降;去卵巢术后各组大鼠骨组织体积、骨小梁体积分数、骨小梁厚度、骨小梁宽度、骨小梁数目、骨小梁模块因素下降明显,骨小梁分离度升高明显;适当剂量蒙药蓝刺头可以明显提高大鼠骨组织体积、骨小梁体积分数、骨小梁厚度、骨小梁宽度、骨小梁数目、骨小梁模块因素,明显降低骨小梁分离度;其具明显抗PMOP作用。结论蒙药蓝刺头对PMOP模型大鼠具显著抗骨质疏松作用;蒙药蓝刺头能显著促进模型大鼠骨组织成骨分化、骨形成;降低PMOP模型大鼠骨代谢高转换指标,抑制骨吸收。
Objective To confirm the preventive and therapeutic effect of Mongolian medicine echinops sphaerocephalus L.on postmenopausal osteoporosis(PMOP).This paper provides experimental basis for Mongolian medicine based on the classical theory of warming and tonifying essence-Qingzhenheyi-bone-fixing and bone-strengthening.It also reveals the theory and therapeutic effect on PMOP and provides ideas for the modernization of Mongolian medicine.Methods Grouping:846-month SPF-grade healthy female sterile rats were randomly divided into 7 groups:sham group,ovariectomized osteoporosis model group(OVX),Echinops-H group,Echinops-M group,Echinops-L group,Herba Epimedii control group(HEP),and Western medicine control group Co-Q10 group(Co-Q10).Modeling:Postmenopausal osteoporosis model was established by bilateral ovariectomy(castration).Vaginal epithelial keratosis test,bone mineral density test,and uterine pathology were used to verify the success of the model.Drug administration:90 days after the operation,rats in the model group and sham-operation group received intragastric administration of normal saline.Rats in the rest groups received intragastric administration of corresponding drugs.Sample collection:Samples were collected after the last gastric perfusion.The right femur of the rats was removed,labeled,and fixed in 4%polyformaldehyde solution.Indicators:Bone mineral density(BMD)of the right femur(proximal femur BMD and total femur BMD)was measured with bone mineral density instrument.Bone microstructural morphometry was examined with micro-CT.Results BMD decreased significantly in all groups after ovariectomy,which was characteristic by decreased cancellous and cortical bone,thinned cortical bone,and decreased BMD.Bone tissue volume,trabecular volume fraction,trabecular thickness,trabecular width,trabecular number,and trabecular module decreased significantly,but the trabecular segregation increased significantly,in all groups after ovariectomy.Appropriate dosage of Mongolian medicine echinops sphaerocephalus L.significantly increased the volume of bone tissue,the volume fraction of bone trabecula,the thickness of bone trabecula,the width of bone trabecula,the number of bone trabecula,and the modular factors of bone trabecula,and significantly reduced the trabecular segregation.The drug had obvious anti-PMOP effect.Conclusion Mongolian medicine echinops sphaerocephalus L.has significant anti-osteoporosis effect on PMOP model rats.Mongolian medicine echinops sphaerocephalus L.significantly promotes osteogenic differentiation and bone formation in PMOP model rats,reduces the high bone metabolic conversion index,and inhibits bone resorption in PMOP model rats.
作者
赵军
董重阳
师建平
刘晋
党赢
常虹
海日
李婧
ZHAO Jun;DONG Chongyang;SHI Jianping;LIU Jin;DANG Ying;CHANG Hong;HAI Ri;LI Jing(Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine,Hohhot 010020;School of Graduate,Nanjing University of Traditional Chinese Medicine,Nanjing 210023;Inner Mongolia Medical University College of Traditional Chinese Medicine,Hohhot 010110;Department of Traditional Chinese Medicine,Hospital Affiliated to Inner Mongolia Medical University,Hohhot 010050,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2020年第7期972-977,共6页
Chinese Journal of Osteoporosis
基金
国家中医药管理局中医药传承与创新“百千万人才工程”项目(J20184832009)
第四批全国中医(临床、基础)优秀人才研修项目(J20184832009)
内蒙古医科大学“科技百万工程”联合项目[YKD2017KJBW(LH)064]
内蒙古自治区草原英才创新团队“中医治未病”项目(CYYCTD2018)。
关键词
绝经后骨质疏松
蒙药蓝刺头
补暖补精-清镇赫依-固骨质壮骨
骨组织形态学
骨密度
postmenopausal osteoporosis
Mongolian medicine echinops sphaerocephalus L.
warming and tonifying essence-Qingzhenheyi-bone fixing and bone strengthening
bone tissue morphology
bone mineral density
