摘要
【目的】了解储粮害虫赤拟谷盗Tribolium castaneum(Herbst)成虫肠道细菌群落组成及多样性。【方法】利用Illumina MiSeq高通量测序技术对3个地理种群的赤拟谷盗雄虫肠道细菌16S rDNA的V3-V4变异区进行测序分析。【结果】从赤拟谷盗肠道样本中共测得465293条reads,聚类为113个OTU,共注释到8门、15纲、33目、52科和79属的细菌。其中,优势菌门包括变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)等,优势菌属包括假单胞菌属(Pseudomonas)、肠杆菌科未鉴定属(Unclassified-f-Enterobacteriaceae)、不动杆菌属(Acinetobacter)、肠球菌属(Enterococcus)和根瘤菌属(Rhizobium)等。3个地理种群的赤拟谷盗肠道细菌的Shannon指数、Simpson指数、Ace指数和Chao指数分别为1.5-1.7、0.3、79.7-133.2和76.4-85.2。【结论】不同地理种群的赤拟谷盗成虫的肠道细菌群落组成存在显著差异。
[Objectives]To reveal the community structure and diversity of the intestinal bacteria of Tribolium castaneum.[Methods]The Illumina MiSeq high-throughput sequencing platform was used to sequence the V3-V4 regions of the bacterial 16S rDNA gene in the intestine of adult male T.castaneum from three geographic populations.[Results]A total of 465293 reads and 113 OTUs were obtained and annotated into 8 phyla,15 classes,33 orders,52 families and 79 genera.The most common phyla were the Proteobacteria,Firmicutes,and Actinobacteria.The most abundant genera were Pseudomonas,unclassified-f-Enterobacteriaceae,Acinetobacter,Enterococcus and Rhizobium.The Shannon,Simpson,Ace and Chao indices were 1.5-1.7,0.30,79.7-133.2 and 76.4-85.2,respectively.[Conclusion]Bacterial community structure and diversity differ among different geographic populations of T.castaneum.
作者
王争艳
王洋
何梦婷
鲁玉杰
WANG Zheng-Yan;WANG Yang;HE Meng-Ting;LU Yu-Jie(Henan University of Technology,School of Food Science and Technology,Zhengzhou 450001,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2020年第3期617-622,共6页
Chinese Journal of Applied Entomology
基金
国家自然科学基金项目(31601890)
河南工业大学河南省省属高校基本科研业务费专项资金(2016XTCX01)
河南省科技攻关项目(202102110059)。
