摘要
目的:探析五子衍宗丸对帕金森病(PD)小鼠的神经保护及抗炎作用。方法:24只C57BL/6雄性小鼠随机分为模型组和五子衍宗丸组,每组12只。腹腔注射MPTP建立小鼠PD模型,同时模型组灌胃0.9%氯化钠溶液,五子衍宗丸组灌胃五子衍宗丸溶液,持续2周。采用旷场和步态实验观察小鼠行为学表现;免疫组织化学染色观察中脑黑质TH阳性神经元;ELISA检测脑组织中细胞因子IL-6、TNF-α、IL-1β、IL-10水平。结果:与模型组比较,五子衍宗丸组小鼠移动总距离及平均速度均显著增加(P<0.01),休息时间和脚步角度显著减少(P<0.05),自主活动量增多;形态学显示中脑黑质TH阳性细胞数显著增多(P<0.05);炎性因子IL-1β、IL-6、TNF-α水平显著降低(P<0.05,P<0.01),抗炎因子IL-10水平显著升高(P<0.01)。结论:五子衍宗丸可明显改善PD小鼠的行为学,保护黑质多巴胺能神经元,其作用机制可能与抑制脑内炎症反应有关。
Objective:To explore the neuroprotective and anti-inflammatory effect of Wuzi Yanzong Pills(WYP)in MPTP-induced Parkinson’s disease(PD)mice.Methods:Twenty-four C57 BL/6 male mice were randomly divided into the model group and WYP group,12 in each group.Mice PD model was established by intraperitoneal injection of MPTP for one week.At the same time,the model group was given 0.9%sodium chloride solution,the WYP group was given WYP solution,lasting two weeks.The animal behavior was evaluated by open field and gait.TH^+ neuron in the substantia nigra of the midbrain was measured by immunohistochemistry.The levels of cytokine IL-6,IL-10,IL-1β,and TNF-α in the extract of the brain were detected by ELISA.Results:Compared with the model group,the total distance and average speed of movement in the WYP group increased significantly(P<0.01),while the rest time and step angle decreased obviously(P<0.05),and the amount of autonomous activity increased.In substantia nigra of bran,TH^+ neuron increased significantly after WYP intervention(P<0.05).At the same time,the levels of inflammatory factors IL-1β,IL-6,and TNF-α decreased significantly(P<0.05,P<0.01),while anti-inflammatory factor IL-10 increased significantly(P<0.01).Conclusion:WYP can significantly improve the behavior of PD mice and protect dopaminergic neurons,which may be related to the anti-inflammatory effect of WYP.
作者
李艳荣
樊慧杰
柴智
孙梦瀛
刘建春
肖午帅
尉杰忠
李艳花
张波
肖保国
马存根
LI Yan-rong;FAN Hui-jie;CHAI Zhi;SUN Meng-ying;LIU Jian-chun;XIAO Wu-shuai;YU Jie-zhong;LI Yan-hua;ZHANG Bo;XIAO Bao-guo;MA Cun-gen(The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis/Neurobiology Research Center,Shanxi University of Chinese Medicine,Jinzhong 030619,China;institute of Brain Science,Shanxi Datong University,Datong 037009,China;Department of Traditional Chinese Medicine,Shanxi Health Vocational College,Jinzhong 030619,China;Institute of Neurology,Huashan Hospital,Fudan University,Shanghai 200025,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第7期3623-3626,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81102552,No.81473577,No.81703978)
山西省重点研发计划项目(No.201803D31091,No.201803D31209)
山西省青年拔尖人才支持计划项目(No.晋组办字[2019]35号文)
山西省自然科学基金项目(No.201901D111334)
山西省国际科技合作项目(No.201703D421016)
山西省回国留学人员科研资助重点项目(No.2014-7,No.2017-129)
山西省留学人员科技活动项目择优资助项目(No.晋财社[2017]19号)
山西省高等学校科技创新计划项目(No.2019L0724)
基于炎症反应的重大疾病创新药物山西省重点实验室开放课题资助项目(No.SXIDL-2018-04)
山西中医药大学中西医结合防治神经系统疾病研究科技创新团队(No.2018TD-012)
药用资源与天然药物化学教育部重点实验室开放课题(No.2019004)。
关键词
五子衍宗丸
帕金森病
行为学
黑质
抗炎作用
神经保护
Wuzi Yanzong Pills
Parkinson’s disease
Ethology
Substantia nigra
Anti-inflammatory effect
Neuroprotection
