摘要
目的:以人正常肝细胞株(L-02细胞)和昆明小鼠为实验对象,研究白杨素衍生物(CD)的抗氧化活性。方法:分别用不同浓度CD(2.5、5、10、20、25μmol·L-1)作用L-02细胞24 h后,采用MTT法检测细胞活力。将L-O2细胞分对照组、H2O2模型组和CD治疗组,采用EUSA法检测CD对H2O2损伤的L-O2细胞中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化物酶(CAT)活性和丙二醛(MDA)含量的影响。昆明小鼠随机分为对照组、H2O2模型组和CD治疗组(5、10 mg·kg-1),实施腹腔注射药品,眼眶取血,随后断头处死小鼠,取肝脏组织。检测小鼠血液和肝脏组织中GSH-Px、SOD、CAT的活性及MDA含量。结果:CD的浓度为2.5~5.0μmol·L-1时,对L-O2细胞的活力无显著影响,因此选择2.5μmol·L-1作为CD的保护浓度。与H2O2模型组相比,CD治疗组中L-O2细胞的GSH-Px、SOD、CAT的活性显著上升,丙二醛含量显著下降;小鼠给药浓度为5 mg·kg-1时,与模型组相比,CD治疗组小鼠血液中GSH-Px、SOD和CAT活性分别升高了108.41%、54.00%、74.29%,丙二醛含量下降47.01%。小鼠肝脏组织中GSH-Px、SOD和CAT活力比模型组分别增加了44.91%、45.17%和55.21%,同时丙二醛含量下降了54.00%。结论:CD对H2O2损伤的L-O2细胞和组织具有保护作用,且表现为浓度依赖性,其潜在机制可能是提高细胞和组织抗氧化能力,从而减轻不良氧化应激反应。
Objective:To investigate the antioxidant activities of chrysin derivative(CD)by using the L-O2 cell line and KM mice. Methods:L-O2 cells were treated with different concentrations of CD(2.5,5,10,20,25 μmol·L-1)for 24 h,and the cell viability was measured using MTT assay. L-O2 cells were divided into control group,H2O2 model group and CD treatment groups. The activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT)and the level of malondialdehyde(MDA)in L-O2 cells were detected by ELISA. KM mice were randomly divided into control group,H2O2 model group and CD treatment groups(5 mg·kg-1,10 mg·kg-1)and were administrated with the control or CD at different concentrations intraperitoneally. Blood was collected from orbital and the liver tissues were collected after r decapitation. The activities of GSH-Px,SOD,GSH-Px,CAT and the level of MDA in both blood and liver tissues were detected by ELISA. Results:CD at the concentrations from 2.5~5.0 μmol·L-1 showed no significant effect on the viability of L-O2,therefore 2.5 μmol·L-1 was used as the protective concentration of CD. Compared with the H2O2 model group,the activities of GSH-Px,SOD,CAT of L-O2 cells in the CD treated groups increased significantly,while the level of MDA was significantly decreased. When the dosage for the mice was 5 mg·kg-1,comparing with the model group,the activities of GSH-Px,SOD,CAT in CD-treated group mice blood increased by 108.41%,54.00% and 74.29%,respectively,while the level of MDA decreased by 47.01%. The activities of GSH-Px,SOD and CAT in mice liver tissues increased by 44.91%,45.17% and55.21%,respectively,and the level of MDA decreased by 54.00%. Conclusion:CD has a protective effect on H2O2-injured L-O2 cells and tissues in a dose-dependent manner,and the underlying mechanism may be the increasing of antioxidant capacity of cells and tissues,thereby reducing the adverse oxidative stress.
作者
李红侠
吴超
钱玉梅
王静
刘雪亚
LI Hong-xia;WU Chao;QIAN Yu-mei;WANG Jing;LIU Xue-ya(School of Life and Food Engineering,Suzhou University,Suzhou 234000,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2020年第6期1005-1010,共6页
Chinese Journal of Pharmaceutical Analysis
基金
宿州学院自然科学重点项目(2017yzd11)
安徽省自然科学基金项目(1908085MC100)。
