摘要
藤黄节杆菌(Arthrobacter luteus,A.luteus)ATCC 21606是一种革兰氏阳性短杆状的放线菌。该菌分泌的溶菌酶(Lyticase)能够有效裂解酵母细胞壁,同时能分泌限制性核酸内切酶AluⅠ和热稳定的黄嘌呤氧化酶,但目前还没有该菌株的全基因组序列相关的报道。本研究首先通过对A.luteus ATCC 21606菌株的基因组进行高通量测序;再利用SOAPdenovo、GeneMarks等软件对基因组进行组装和组分分析;接着与COG、GO、KEGG、NR、Swiss-Prot和CAZy数据库比对进行基因功能注释;并利用antiSMASH软件进行次级代谢产物合成基因簇预测;最终得到大小为4209480 bp的全基因组序列,GC含量为74.68%,共预测到编码基因3741个。基因序列已提交至美国国立生物技术信息中心(NCBI)的GenBank数据库,登录号为RQIK000-00000。本研究首次报道了A.luteus ATCC 21606的全基因组序列,为后续该菌株的功能基因、代谢产物合成途径及比较基因组学等相关研究提供基础。
Arthrobacter luteus(A.luteus)ATCC 21606 is a gram stain positive Actinobacteria.Lyticase secreted by this bacterium can effectively lyse yeast cells.A.luteus ATCC 21606 also can produce AluⅠ,a kind of restriction endonuclease and a thermostable xanthine oxidase.At present,the complete genome sequence of A.luteus ATCC21606 has not been reported yet.Firstly,the A.luteus ATCC21606 strain genome was sequenced using Illumina High-throughput sequencing platform,and assembled by SOAPdenovo softwares.The gene components analyzed by GeneMarks.Then,the predicted coding genes were compared with the databases of COG,GO,KEGG,NR,Swiss-prot and CAZy for gene function annotation,and genetic cluster prediction of secondary metabolites synthesis was analyzed using antiSMASH software.Finally,the result was that the whole genome size of A.luteus is 4209480 bp,GC content is 74.68%and encodes 3741 protein genes.The genome sequence was deposited in the GenBank database under the accession number RQIK00000000.In this study,the whole genome sequence of A.luteus ATCC 21606 was first reported,which provided a basis for the subsequent studies on functional genes,metabolic product synthesis pathways and comparative genomics of the strain.
作者
谷璐婷
李凌彦
孔道春
易发平
Gu Luting;Li Lingyan;Kong Daochun;Yi Faping(Department of Biochemistry and Molecular Biology,Chongqing Medical University,Chongqing,400016;National Laboratory of Protein and Plant Genes,College of Life Sciences,Peking University,Beijing,100871)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第5期2071-2078,共8页
Genomics and Applied Biology
基金
国家自然科学基金重点项目(31730022)
国家自然科学基金国际合作项目(NSFC-ISF,31661143032)共同资助
科技部国家重点研发计划(2016YFA0500300)。
