摘要
目的:优化沙棘总黄酮提取工艺,获取沙棘总黄酮最佳提取工艺条件及技术参数。方法:采用Box-Benhnken中心组合设计,以提取物中总黄酮含量和总黄酮提取率的总权重评分值为评价指标,对提取液料比、乙醇浓度、提取时间3个影响因素进行考察,以Design-Expert 8.05软件对试验数据作统计处理。结果:优化得到最佳工艺条件:9倍量70%乙醇,回流提取2次,每次2 h。结论:优选得到的工艺稳定可行,可作为沙棘提取提供参考依据。
Objective:To optimize the extraction of total flavonoids in Hippophae rhamnoides L.and obtain the optimal extraction conditions and technical parameters of total flavonoids in Hippophae rhamnoides L.Methods:With the content and the extraction rate of total flavonoids in Hippophae rhamnoides L.as the indices,the effects of three factors including the solvent-solid ratio,ethanol concentration and extraction time were explored by Box-Behnken central composite.Design-Expert 8.05 software was used to statistically process the experimental data.Results:The optimal extraction conditions of Hippophae rhamnoides L.were as follows:the ethanol concentration was 90%,the extracting time was two hours and the solvent-solid ratio was 9.Conclusion:The optimized extraction process of total flavonoids is stable and feasible,and the result can provide theoretical guidance for the extraction of total flavonoids in Hippophae rhamnoides L.
作者
彭明
Peng Ming(Department of Pharmacy,Wuhan Mental Health Center,Wuhan 430012,China)
出处
《中国药师》
CAS
2020年第7期1322-1326,共5页
China Pharmacist
关键词
沙棘
总黄酮
提取工艺
星点设计-响应面法
Hippophae rhamnoides L.
Total flavonoids
Extraction
Central composite design and response surface method
