摘要
目的:探讨肺鳞癌成纤维细胞生长因子受体1(fibroblast growth factor receptor 1,FGFR1)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、磷酸酶及张力蛋白同源基因(phosphatase and tensin homolog deleted on chromosome ten,PTEN)蛋白表达与临床病理特征及预后的相关性。方法:收集南京医科大学第一附属医院2011—2013年临床病理资料完整的肺鳞癌标本149例,制作石蜡组织芯片,采用免疫组织化学MaxVision法检测肿瘤细胞FGFR1、bFGF、PTEN蛋白表达水平,并通过电话和门诊对患者进行术后随访。结果:①肿瘤细胞FGFR1阳性率为51.7%(77/149),其表达与吸烟史(P=0.019)、淋巴结转移(P<0.001)及分化程度(P<0.001)相关;bFGF阳性率为61.7%(92/149),其表达与淋巴结转移、T分期相关(P<0.05);PTEN阴性表达率为57.0%(85/149),其表达缺失仅与分化程度相关(P<0.05)。FGFR1表达与b FGF表达、PTEN表达缺失存在相关性(P<0.05)。②单因素生存分析显示,肿瘤细胞FGFR1、bFGF阳性与无复发生存期(relapse free survival,RFS)和总生存期(overall survival,OS)均呈显著相关(P<0.05);PTEN表达缺失与预后无明显相关性。③Cox多因素生存分析显示,FGFR1表达情况是肺鳞癌患者RFS和OS的独立预后因子(P<0.05)。结论:肺鳞癌FGFR1阳性表达与肿瘤低分化、有淋巴结转移及吸烟史显著相关,且与bFGF表达及PTEN表达缺失显著相关,肿瘤细胞FGFR1阳性提示患者生存期较短,是肺鳞癌患者的独立预后因素。
Objective:This study aims to investigate the expression of fibroblast growth factor receptor 1(FGFR1),basic fibroblast growth factor(bFGF)and phosphatase and tensin homolog deleted on chromosome ten(PTEN)in lung squamous cell carcinoma(SCC)and its correlation with clinicopathological features and prognosis.Methods:All of 149 cases of lung SCC with complete clinicopathological data from 2011 to 2013 were collected from the First Affiliated Hospital of Nanjing Medical University to produce tissue microarrays.The expression of FGFR1,bFGF and PTEN was detected by MaxVision immunohistochemical method.Postoperative follow-up of patients was taken by telephone and outpatient service.Results:①In lung SCC,the positive rate of FGFR1,bFGF were 51.7%(77/149)and 61.7%(92/149)respectively.The expression of FGFR1 was significantly associated with smoking(P=0.019),lymph node metastasis(P<0.001)and differentiation of tumor(P<0.001).The expression of bFGF was correlated with lymph node metastasis and T stage(P<0.05).The negative rate of PTEN was 57.0%(85/149).The loss of PTEN expression was related to differentiation(P<0.05).In addition,FGFR1 expression was significantly associated with bFGF expression or PTEN expression(P<0.05).②Univariate survival analysis showed that the expression of bFGF and FGFR1 were significantly correlated with the relapse free survival(RFS)and overall survival(OS)(P<0.05).There was no significant correlation between the loss expression of PTEN and RFS/OS.③The Cox multi-factor survival analysis showed that FGFR1 expression was an independent prognostic factor for RFS and OS(P<0.05).Conclusion:The expression of FGFR1 in lung SCC was significantly correlated with poor differentiation,lymph node metastasis,smoking history,bFGF expression and loss of PTEN expression.Patients with FGFR1 positive expression had shorter survival time than those without FGFR1 expression.FGFR1 was an independent prognostic factor for SCC.
作者
何晓蓉
宋国新
周晋星
李红霞
HE Xiaorong;SONG Guoxin;ZHOU Jinxing;LI Hongxia(Department of Pathology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029;Department of Pathology,the Affiliated Wuxi Municipal Maternal and Child Health Care Hospital of Nanjing Medical University,Wuxi 214002,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第6期839-845,共7页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金青年基金(81201703)。
关键词
肺鳞癌
FGFR1
BFGF
PTEN
组织芯片
病理特征
预后
lung squamous cell carcinoma
FGFR1
bFGF
PTEN
tissue microarray
pathological features
prognosis
