摘要
目的:观察健脾疏肝降脂方对高脂饮食诱导的非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)肥胖小鼠的治疗效果。方法:将72只小鼠随机分为模型组,健脾疏肝降脂方高剂量组、中剂量组、低剂量组,壳脂胶囊对照组和空白组。采用饲养高脂饲料及皮下注射体积分数5%CCl4复制NAFLD肥胖模型小鼠,分别以相应药物灌胃6周,检测血清中三酰甘油(triacylglycerol,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein,HDL)、低密度脂蛋白(low density lipoprotein,LDL)的水平,光镜下观察肝组织的脂肪变程度。结果:①与空白组比较,模型组小鼠肝指数及血清TC、LDL均明显升高(P<0.01)。与模型组比较,各给药组血脂指标均明显降低(P<0.05);其中,健脾疏肝降脂方高剂量组、中剂量组明显优于壳脂胶囊对照组(P<0.05)。②各给药组光镜下病理组织改变均较模型组明显,其中健脾疏肝降脂方高剂量组、中剂量组小鼠肝细胞脂肪变程度减轻明显优于低剂量组(P<0.05)。结论:健脾疏肝降脂方可明显改善NAFLD肥胖小鼠的脂肪变程度,其作用机理可能与调节机体内脂肪代谢、改善肝小叶结构有关。
Objective:To observe the therapeutic effect of Jianpi Shugan Jiangzhi Recipe on obese mice with non-alcoholic fatty liver disease(NAFLD)induced by high-fat diet.Methods:72 mice were randomly divided into model group,Jianpi Shugan Jiangzhi Recipe high-dose group,middle-dose group,low-dose group,shell fat capsule control group and blank group.Using high-fat feed and subcutaneous injection volume fraction of 5%CCl4 to replicate NAFLD obese model mice,the rats were gavaged with corresponding drugs for 6 weeks to detect serum triacylglycerol(TG),total cholesterol(TC),The levels of high density lipoprotein(HDL)and low density lipoprotein(LDL)were observed under the light microscope to observe the degree of fatty change in liver tissue.Results:①Compared with the blank group,the liver index,serum TC and LDL of the model group were significantly increased(P<0.01).Compared with the model group,the blood lipid index of each administration group was significantly reduced(P<0.05);among them,Jianpi Shugan Jiangzhi Recipe high-dose group and middle-dose group were significantly better than the shell fat capsule control group(P<0.05).②The pathological changes of each drug administration group under light microscope were more obvious than the model group.Among them,Jianpi Shugan Jiangzhi Decoction′s high-dose group and middle-dose group had significantly lower hepatic cell fatity than the low-dose group(P<0.05).).Conclusion:Jianpi Shugan Jiangzhi Recipe can significantly improve the degree of steatosis in NAFLD obese mice,and its mechanism may be related to the regulation of body fat metabolism and the improvement of liver lobular structure.
作者
巴明玉
娄永强
禄保平
BA Mingyu;LOU Yongqiang;LU Baoping(The First Affiliated Hospital to Henan University of Chinese Medicine,Zhengzhou Henan China 450000;Tongxu County Hospital of Chinese Medicine,Tongxu Henan China 475400;Henan University of Chinese Medicine,Zhengzhou Henan China 450046)
出处
《中医学报》
CAS
2020年第8期1723-1726,共4页
Acta Chinese Medicine
基金
河南省教育厅科学技术研究重点项目(13A3-60611)。
关键词
健脾疏肝降脂方
非酒精性脂肪肝
肥胖
肝指数
血脂
三酰甘油
总胆固醇
高密度脂蛋白
低密度脂蛋白
小鼠
Jianpi Shugan Jiangzhi Recipe
nonalcoholic fatty liver
obesity
liver index
blood lipids
triacylglycerol
total cholesterol
high density lipoprotein
low density lipoprotein
mice
