摘要
从新鲜生牛奶中分离筛选产丙酸较高的菌株,经形态学特征、生理生化及糖发酵试验、16S rDNA基因序列同源性分析以及多位点序列分型(multilocus sequence typing)等实验鉴定该菌株,分析了其对不同碳源的利用率及产酸情况,并从溶血性试验及抗生素抗性试验方面来评估该菌株的安全性。结果表明,从4批次样品中共分离获得54株能产丙酸的菌株,其中一株菌的丙酸产量达到7.38 g/L,为费氏丙酸杆菌(Propionibacterium freudenreichii)。比较了葡萄糖和甘油对其生长的影响,发现其对葡萄糖的利用率大于对甘油的利用率,在含葡萄糖的培养基中于30℃厌氧培养120 h后丙酸产量达到7.38 g/L。该菌株无溶血性,对卡那霉素等氨基糖苷类抗生素有耐药性,对氨苄西林、万古霉素、氯霉素、克林霉素、四环素敏感,对红霉素中介。综上,费氏丙酸杆菌B1具有一定的潜在应用价值。
The high propionate-producing strains were isolated and screened from fresh raw milk,and experiments such as morphological characteristics,physiological and biochemical and sugar fermentation tests,16 S rDNA gene sequence homology analysis,and multilocus sequence typing were performed to identify the strain.The utilization rate and acid production of different carbon sources were analyzed,and the safety of the strain was evaluated from the aspects of hemolysis test and antibiotic resistance test.Results showed that:A total of 54 strains capable of producing propionic acid were isolated from 4 batches of samples,and the propionic acid yield of one strain reached 7.38 g/L,which was Propionibacterium freudenreichii.The effects of glucose and glycerol on their growth were compared,and it was found that the utilization of glucose was greater than the utilization of glycerol.The yield of propionic acid reached 7.38 g/L after anaerobic culture at 30℃for 120 h in glucosecontaining medium.The strain is non-hemolytic,resistant to aminoglycoside antibiotics such as kanamycin,sensitive to ampicillin,vancomycin,chloramphenicol,clindamycin,tetracycline,and mediated by erythromycin.In summary,Propionibacterium freudenreichii B1 has certain potential application value.
作者
范小飘
高文文
李欣芮
赵桉
赵鹏昊
尚佳萃
赵乐
周雪
孟祥晨
FAN Xiao-piao;GAO Wen-oen;LI Xin-rui;ZHAO An;ZHAO Peng-hao;SHANG Jia-cui;ZHAO Le;ZHOU Xue;MENG Xiang-chen(Key Laboratory of Daiy Science,Ministry of Education,School of Food Science,Northeast Agricultural University,Harbin 150038,China)
出处
《食品工业科技》
CAS
北大核心
2020年第15期106-113,共8页
Science and Technology of Food Industry
基金
国家重点研发计划(2017YFD0400304)。
关键词
费氏丙酸杆菌
分离与鉴定
生物学特性
抗生素抗性
Propionibacterium freudenreichii
isolation and identification
biological characteristics
antibiotic resistance
