摘要
目的比较甲苯胺红不加热血清试验(TRUST)和酶联免疫吸附试验(ELISA)在检测梅毒螺旋体中的灵敏度、特异度差别。方法对2018年1月—2019年6月本院收集的1500例疑似梅毒患者血清标本进行检测,分别用ELISA、TRUST法以及梅毒螺旋体明胶凝集试验(TPPA)检测梅毒螺旋体抗体,评估其应用价值。结果 1500例疑似患者中梅毒确诊患者为1093例,排除患者407例。ELISA法检测的灵敏度、特异度、阳性预测值、阴性预测值分别为98.08%、96.07%、98.53%、94.09%,TRUST法检测分别为92.04%、87.22%、95.08%、80.32%。ELISA各项指标均高于TRUST,且差异有统计学意义(P<0.05)。结论 TRUST检测的是梅毒非特异性抗体,ELISA检测的是梅毒特异性抗体,临床上筛查梅毒应结合两种方法,有效地减少漏诊和误诊。
Objective To compare the difference of sensitivity and specificity of toluidine red unheated serum test(TRUST) and enzyme-linked immunosorbent assay(ELISA) in detection of Treponema pallidum.Methods From January 2018 to June 2019, 1500 cases of blood samples collected from the patients with suspected syphilis was used to detect Treponema pallidum. Treponema pallidum antibodies were detected by ELISA, TRUST and TPPA method, respectively, and their application value was evaluated.Results Of the 1500 suspected cases, 1093 were diagnosed with syphilis and 407 were excluded. The sensitivity, specificity, positive predictive value and negative predictive value of ELISA assay were 98.08%, 96.07%, 98.53%, 94.09%, respectively. Those of the trust assay were 92.04%, 87.22%, 95.08%, 80.32%, respectively. Indexes of ELISA were higher than TRUST, and the difference was statistically significant(P<0.05).Conclusions TRUST is a non-specific antibody test method for syphilis and ELISA is a specific antibody test method for syphilis. Clinical screening of syphilis should combine the two methods to effectively reduce missed diagnosis and misdiagnosis.
作者
林明春
杨永红
朱小灯
石伟
LIN Ming-chun(Guizhou provincial orthopedic hospital,Guiyang,Guizhou,550007,China.)
出处
《齐齐哈尔医学院学报》
2020年第10期1219-1220,共2页
Journal of Qiqihar Medical University
