摘要
目的观察复方芪鹰颗粒对糖尿病神经病理性疼痛(DNP)大鼠的干预作用及对PI3K/Akt信号通路的影响。方法选择15只雄性SD大鼠作为对照组,另选择35只雄性SD大鼠通过腹腔注射链脲佐菌素(STZ)方法建立DNP模型。将造模成功的30只大鼠随机分为DNP组和干预组,干预组大鼠给予复方芪鹰颗粒3 g/kg灌胃,对照组和DNP组大鼠给予等量的生理盐水灌胃,均每日1次,连续14 d。检测建模前后及灌胃7 d和14 d后各组大鼠机械性缩足反应阈值(PWMT)和热缩足潜伏期(TWL),灌胃14 d后分别通过尼氏染色和TUNEL染色检测各组大鼠背角脊髓神经元形态、凋亡情况,采用Western blot法检测各组大鼠背角脊髓中PI3K、p-PI3K、Akt1和p-Akt1蛋白表达水平。结果与建模前比较,建模后DNP组和干预组大鼠的PWMT均显著降低(P均<0.05),TWL均显著缩短(P均<0.05)。灌胃7 d和14 d后,DNP组大鼠的PWMT均显著低于对照组(P均<0.05),TWL均显著短于对照组(P均<0.05);而干预组大鼠的PWMT均显著高于DNP组(P均<0.05),TWL均显著长于DNP组(P均<0.05)。DNP组大鼠可见背角萎缩和神经元病变坏死,尼氏体数量减少或消失;干预组可见清晰的尼氏体并且尼氏体数量较DNP组有所恢复。DNP组大鼠的背角脊髓神经元凋亡指数显著高于对照组(P<0.05),干预组的凋亡指数显著低于DNP组(P<0.05)。DNP组的p-PI3K/PI3K和p-Akt1/Akt1表达水平均显著高于对照组(P均<0.05),干预组的p-PI3K/PI3K和p-Akt1/Akt1表达水平均显著低于DNP组(P均<0.05)。结论复方芪鹰颗粒可通过抑制背角脊髓中PI3K/Akt通路蛋白的磷酸化,保护尼氏体,减少神经元凋亡而起到治疗DNP的作用。
Objective It is to observe the intervention effect of compound Qiying granule on diabetic neuropathic pain(DNP)in rats and its effect on PI3K/Akt signaling pathway.Methods Fifteen male SD rats were selected as the control group,and another 35 male SD rats were selected to establish DNP models by intraperitoneal injection of streptozotocin(STZ).Thirty successful model rats were randomly divided into DNP group and intervention group.The rats in the intervention group were given 3 g/kg compound Qiying granules by gavage,and the rats in the control group and DNP group were given the same amount of normal saline by gavage,all once a day for 14 consecutive days.The paw withdrawal mechanical threshold(PWMT)and thermal withdrawal latency(TWL)were measured before and after modeling,and on the 7th and 14th day after intervention.The morphology and apoptosis of spinal cord neurons in each group were detected by Nissl staining and TUNEL staining.The expression levels of PI3K,p-PI3K,Akt1 and p-Akt1 were detected by Western blot.Results Compared with before modeling,the PWMT of the rats in the DNP group and the intervention group was significantly reduced and the TWL was significantly shortened after modeling(P<0.05).After gavage for 7 and 14 days,the PWMT of the rats in the DNP group was significantly lower and the TWL was significantly shorter than that of the control group(all P<0.05),while the PWMT of the intervention group rats were all significantly higher and TWL was significantly longer than that of the DNP group(all P<0.05).The rats in the DNP group showed atrophy of the dorsal horn and neuronal necrosis,and the number of Nissl bodies decreased or disappeared;the intervention group saw clear Nissl bodies and the number of Nissl bodies recovered compared with the DNP group.The apoptosis index of dorsal horn spinal cord neurons in the DNP group was significantly higher than that of the control group(P<0.05),and the apoptosis index of the intervention group was significantly lower than that of the DNP group(P<0.05).The expression levels of p-PI3K/PI3K and p-Akt1/Akt1 in the DNP group were significantly higher than those in the control group(both P<0.05),and the expression levels of p-PI3K/PI3K and p-Akt1/Akt1 in the intervention group were significantly lower than those in the DNP group(all P<0.05).Conclusion Compound Qiying granule can inhibit the phosphorylation of PI3K/Akt pathway protein in the spinal cord,protect Nissl bodies,reduce neuronal apoptosis and thus to treat for DNP.
作者
阿布都沙拉木·阿布都热衣木
刘涛
任海迪
胡晓灵
Abudu Shalamu·Abudu Reyimu;LIU Tao;REN Haidi;HU Xiaoling(The Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830002, Xinjiang, China)
出处
《现代中西医结合杂志》
CAS
2020年第23期2518-2522,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
自治区中医民族医与西医结合研究项目(2016-04-03)。
