摘要
Carfentrazone-ethyl and tribenuron-methyl,the two widely used herbicides for weed control in field crops,frequently cause phytotoxicity to wheat seedlings in the field.In this study,a total of 697 wheat accessions containing three panels were scanned using wheat90 K and 660 K SNP arrays to identify important herbicide resistance loci.Genome-wide association study(GWAS)revealed 329 significant single-nucleotide polymorphisms(SNPs)with phenotypic variance explained(PVE)of 11.3%to 27.6%.Among these SNPs,15 were detected in multiple environments and they were mainly distributed on chromosomes 1 B,2 D,5 B,5 D,6 D,and 7 D.Further analysis indicated that g HR-5 B(467–587 Mb),g HR-7 D(46–52 Mb),and g HR-1 B(517–580 Mb)were important herbicide resistance loci in wheat.Linkage mapping in a bi-parental population detected one QTL(q HR-1 B)with PVE of 7.44%to8.28%.This is reliable locus because its physical position(554–566 Mb)overlapped with g HR-1 B by GWAS in the genome of Chinese Spring.This study provided some herbicide-resistant germplasm and important genetic loci for identifying genes of common wheat.
基金
supported by the National Key Research and Development Program of China(2016YFD0101802)
the National Natural Science Foundation of China(3181101544)
Henan Major Science and Technology Projects(181100110200)
Ten-Thousand Talents Plan(Z04295)of China。
