摘要
目的:对赛北紫堇90%乙醇提取物化学成分进行系统研究,并对分离得到的化合物进行体外细胞增殖抑制作用评价。方法:采用正相硅胶,LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20),ODS柱色谱以及半制备高效液相色谱等分离技术进行分离纯化,运用NMR,MS等波谱方法以及理化性质结合文献数据对分离得到的化合物进行结构鉴定,并采用四甲基唑蓝(MTT)法测定了分离得到的13个化合物对人肝癌HepG2,SMMC-7721细胞的体外抑制活性。结果:从赛北紫堇90%乙醇提取物中分离并鉴定了13个化合物,其结构分别为5-羟基吡啶-2-甲酸乙酯(1),元胡内酯(2),3,4-顺-3,4-二羟基-β-紫罗兰酮(3),megastigmane(4),9-hydroxy-4,7-megastigmadien-3-one(5),blumenol A(6),吲哚-3-羧酸(7),1-methyl-[1,2,4]triazolo[4,3-b][1,2,4]triazin-7-one(8),腺嘌呤(9),烟酰胺(10),2-羟甲基-5-羟基吡啶(11),腺嘌呤核苷(12),β-胡萝卜苷(13)。细胞增殖抑制作用显示化合物3对人肝癌HepG2细胞的半数抑制浓度(IC50)为24. 7μmol·L^-1(阳性药顺铂IC50为4. 8μmol·L^-1),化合物4对人肝癌SMMC-7721细胞的IC50为13. 8μmol·L^-1(阳性药顺铂IC50为5. 4μmol·L^-1)。结论:其中化合物1为一新的天然产物,3~8为首次从紫堇属中分离得到,化合物2,9~12为首次从赛北紫堇中分离得到。化合物3对人肝癌HepG2具有较弱的抑制活性,化合物4对人肝癌SMMC-7721具有中等的抑制活性,其他化合物对上述两种肝癌细胞均没有明显的抑制活性。
Objective:To systematically investigate the chemical constituents of 90% ethanol extract of Corydalis impatiens,and evaluate their inhibitory effect on cell proliferation in vitro. Method: The chemical constituents were isolated and purified by silica gel,Sephadex LH-20,ODS column chromatography and semipreparative high performance liquid chromatography(HPLC). the structures were identified by spectroscopy methods such as NMR and MS,as well as analysis of physicochemical properties and/or comparison with literature data,and the inhibitory activities of 13 compounds on HepG2 and SMMC-7721 cells were measured by methyl thiazolyl tetrazolium(MTT)method. Result: Thirteen known compounds were isolated and identified from 90% ethanol extract of Corydalis impatiens as ethyl-5-hydroxy-2-pyridinecarboxylate(1),coryhumolide(2),3,4-cis-3,4-dihydroxy-β-ionone(3),megastigmane(4),9-hydroxy-4,7-megastigmadien-3-one(5),blumenol A(6),indole-3-carboxy acid(7),1-methyl-[1,2,4]triazolo[4,3-b] [1,2,4]triazin-7-one(8),adenine(9),nicotinamide(10),2-hydroxymethyl-5-pyridinol(11),adenosine(12), and β-daucosterol(13).Cytotoxicities assay showed that the IC50 value of compound 3 for the hepatic cell line HepG2 was 24.7 μmol·L-1(positive control drug cisplatin:4.8 μmol·L^-1),and IC50 value of compound 4 for the hepatic cell line SMMC-7721 was 13.8 μmol·L^-1(positive control drug cisplatin:5.4 μmol·L^-1). Conclusion: Compound 1 was a new natural compound,compounds 3-8 were obtained from genus Corydalis for the first time,and compounds 2,9-12 were isolated from this plant for the first time. Compound 3 exhibited weak inhibitory effect on hepatic cell line HepG2,and compound 4 exhibited moderate inhibitory effect on hepatic cell line SMMC-7721. The other compounds rest of ones exhibited no obvious inhibitory effect on hepatic cell line HepG2 or SMMC-7721.
作者
南泽东
韩广田
李喜安
任华忠
鱼江
王寿峰
郭力
NAN Ze-dong;HAN Guang-tian;LI Xi-an;REN Hua-zhong;YU Jiang;WANG Shou-feng;GUO Li(Chengdu University of Traditional Chinese Medicine(TCM),School of Pharmacy,Southwest Genuine Medicinal Materials Collaborative Innovation Center,State Key Laboralory of Resources Systems Research and Development Utilization of TCM,Chengdu 611137.China;Leshan Vocational&Technical college,Leshan 614000,China;Sichuan University of Science and Engineering,College of Chemical Engineering,Zigong 643000,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第15期163-168,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省科技计划项目(2016JY0087)
乐山职业技术学院博士科研启动基金项目(201606)。
关键词
赛北紫堇
化学成分
结构鉴定
细胞增殖抑制作用
Corydalis impatiens
chemical constituents
structure elucidation
cytotoxic activity
