摘要
目的:探讨穴位注射对气滞血瘀证雌性大鼠血清一氧化氮(NO)、子宫组织血管内皮生长因子(VEGF)、血管内皮生长因子受体2(KDR)表达的影响及可能的作用机制。方法:建立气滞血瘀证雌性大鼠模型,运用免疫组化和RT-PCR法检测空白组、模型组、中药组、穴位注射组大鼠子宫组织VEGF/KDR蛋白及mRNA的表达及血清NO的变化。结果:与空白组比较,模型组大鼠子宫组织VEGF和KDR蛋白及mRNA表达显著降低,NO显著降低;与模型组比较,中药组和穴位注射组子宫组织中VEGF和KDR蛋白及mRNA表达均显著升高,NO明显上升,且2组比较差异无统计学意义。结论:丹红穴位注射能促进气滞血瘀证雌性大鼠血清NO、子宫组织VEGF/KDR的表达,改善子宫组织血流状态,从而达到“祛瘀生新”的作用。
Objective:To investigate the effect of Acupoint Injection on the expression of serum nitric oxide(NO),vascular endothelial growth factor(VEGF)and vascular endothelial growth factor receptor 2(KDR)in uterus of female rats with qi stagnation and blood stasis syndrome and its possible mechanism.Methods:The female rat model of Qi stagnation and blood stasis syndrome was established.The expression of vascular endothelial growth factor(VEGF)/KDR protein and its mRNA in uterus and serum NO were detected by immunohistochemistry and real-time fluorescence quantitative PCR in blank group,model group,traditional Chinese medicine group and acupoint injection group.Result:Compared with model group,NO increased significantly in TCM group and acupoint injection group,and the expressions of VEGF,KDR protein and mRNA in uterine tissue increased significantly,and there was no significant difference between the two groups.Conclusion:Danhong acupoint injection can promote the expression of NO in serum and VEGF/KDR in uterus tissue of female rats with Qi stagnation and blood stasis syndrome,improve the blood flow state of uterus tissue,and thus achieve the effect of“removing blood stasis and generating new blood”.
作者
万荷天一
陈睿
唐也笑
罗小光
WAN He-tian-yi;CHEN Rui;TANG Ye-xiao;LUO Xiao-guang(Guizhou Medical University,Guiyang 550004,China;Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China)
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2020年第7期904-907,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
贵州省科技厅附院联合基金[黔科合LH字(2015)7437号]-刺络、刮痧、穴位注射对女性疾病气滞血瘀证大鼠模型的影响
贵州省中医药管理局[WT2015-13]-刺络拔罐、通络刮痧和穴位注射疗法对气滞血瘀雌性大鼠模型实验研究。
关键词
穴位注射
气滞血瘀证
子宫
VEGF/KDR
血清NO
Acupoint injection
Qi stagnation and blood stasis syndrome
Uterus
VEGF/KDR
Serum NO
