摘要
目的:探讨高危人乳头瘤病毒16型致癌基因E6(HPV16-E6-DNA)载量与宫颈病变程度关系。方法:取集2017年1月-2019年4月本院妇科收治的宫颈病变患者173例临床资料,根据病理学结果分为低度鳞状上皮内瘤变67例(LSIL组)、高度鳞状上皮内瘤变74例(HSIL组)、宫颈癌32例(宫颈癌组),荧光定量PCR技术检测各组宫颈组织标本中HPV16-E6-DNA表达,并分析在不同临床分期(FIGO)、分化程度、病灶大小、肌层浸润程度、病灶类型、淋巴结转移等表达差异。结果:组织中HPV16-E6-DNA载量,宫颈癌组达到>107者占43.8%、105~107占37.5%、<105占18.8%,HSIL组>107占21.6%、105~107占35.1%、<105占43.2%,LSIL组>107占11.9%、105~107占20.9%、<105占67.2%,宫颈癌组HPV16-E6-DNA载量高于LSIL组和HSIL组(P<0.05);宫颈癌组FIGO分期(Ⅲ+Ⅳ期)、肌层浸润>1/2、淋巴结转移阳性标本中HPV16-E6-DNA载量更高(P<0.05),而不同组织分化程度、病灶直径及病灶类型的标本中HPV16-E6-DNA载量未见差异(P>0.05)。结论:在宫颈癌组织中HPV16-E6-DNA载量较LSIL和HSIL患者更高,并与宫颈癌病变不同病理特征有一定关系。
Objective:To investigate the relationship between the load of high-risk human papillomavirus type 16 oncogene E6(HPV16-E6-DNA)and the degree of cervical lesions.Methods:The clinical data of 173 patients with cervical lesions were collected.According to the pathological results,67 patients with low grade squamous intraepithelial neoplasia were in group A,74 patients with high squamous intraepithelial neoplasia were in group B,and 32 cases of cervical cancer patients were in group C.Fluorescent quantitative PCR(RT-PCR)technology was used to detect the expression of HPV16-E6-DNA in cervical lesions tissues of women in the three groups.The differential expression of HPV16-E6-DNA in cervical lesions tissues of women with different clinical stages(FIGO),degree of differentiation,lesion size,degree of myometrial invasion,lesion type,or lymph node metastasis was analyzed.Results:In group C,the percent of HPV16-E6-DNA load in cervical cancer tissues>107,105-107,and<105 were 43.8%,37.5%,and 18.8%,respectively.In group B,the percent of HPV16-E6-DNA load in cervical cancer tissues>107,105-107,and<105 were 21.6%,35.1%,and 43.2%,respectively.In group A,the percent of HPV16-E6-DNA load in cervical cancer tissues>107,105-107,and<105 were 11.9%,20.9%,and 67.2%,respectively.The HPV16-E6-DNA load in cervical cancer tissues of women in group C was significant higher than that of women in group B or C(P<0.05).HPV16-E6-DNA load in cervical cancer tissue of women with FIGO stage(III+IV phase),myometrial invasion>1/2,or lymph node metastasis was significant higher than that of women with FIGO stage(I-II phase)or myometrial invasion≤1/2,or without lymph node metastasis(P<0.05).There was no significant different in HPV16-E6-DNA load of cervical lesion tissues among women with different histological differentiation,different lesion diameters,or different lesion type(P>0.05).Conclusion:The HPV16-E6-DNA load in cervical lesion tissues of women with cervical cancer is higher than that of women with cervical LSIL and HSIL,which is a certain relevant to the pathological features of cervical cancer lesion.
作者
张桂华
张立红
ZHANG Guihua;ZHANG Lihong(Liaocheng Infectious Disease Hospital, Shandong Province,252000)
出处
《中国计划生育学杂志》
2020年第6期918-921,共4页
Chinese Journal of Family Planning
关键词
宫颈病变组织
高危人乳头瘤病毒
致癌基因
蛋白载量
病理特征
Cervical cancer lesions tissue
High-risk human papillomavirus
Oncogene
Protein load
Pathological features
