摘要
以茶树为研究对象,采用茶树茎材切片及染色技术,在生物数码显微镜的普通光和荧光下,对未处理的茶树茎材切片、番红染色茶树茎材切片及吖啶橙染色茶树茎材切片进行比较与分析。结果表明,茶树茎材切片使用吖啶橙染色后在普通光和荧光下确定树龄的效果较好。这种基于数字图像确定茶树树龄的吖啶橙染色法,具有快速、准确、简单和成本低等特点,对大茶树和古茶树活体生长锥取样年轮条的年轮识别具有科学参考意义。
Tea plants were taken as the research objects in this study.Using tea stem slicing and dyeing technologies,the age of tea plants was accurately determined based on the digital images.Under ordinary light and fluorescence of bio-digital microscope,the untreated sections of tea stem,saffron-dyed sections and acridine orange-dyed sections of tea stem were compared and analyzed.The result shows that acridine orange dyeing was the best to determine the age of tea plants.The acridine orange dyeing method combined with digital image analysis had the characteristics of fast,accurate,simple and low cost.In conclusion,the technology also had a scientific reference significance for the identification of annual rings of living large and ancient tea plants.
作者
江燕
黎星辉
浦滇
蓝增全
吴田
JIANG Yan;LI Xinghui;PU Dian;LAN Zengquan;WU Tian(Southwest Landscape Architecture Engineering Research Center of State Forestry Administration,Southwest Forestry University,Kunming 650224,China;Tea Research Institute,Nanjing Agricultural University,Nanjing 210095,China;School of Ecology and Soil and Water Conservation,Southwest Forestry University,Kunming 650224,China;Tea Ecology Research Center,Southwest Green Development Institute,Southwest Forestry University,Kunming 650224,China)
出处
《茶叶科学》
CAS
CSCD
北大核心
2020年第4期492-500,共9页
Journal of Tea Science
基金
国家现代农业产业技术体系建设专项资金(CARS-19)
国家自然科学基金(31470690)
古茶树保护与可持续利用国家创新联盟([2018]96)
云南省教育厅财政厅项目(LY2017-002)。
关键词
茶树茎材切片
染色技术
荧光
年轮
数字图像
stem slices of tea plant
dyeing technology
fluorescence
growth ring
digital image
