摘要
返青期休牧可以有效保护草地生态环境,研究不同休牧期可对休牧措施进行可行性分析,尤其是在三江源区高寒草甸生态系统。土壤微生物碳代谢功能对不同返青休牧期的响应及其与土壤和微生物的作用机制研究,可为评估休牧措施及草地土壤环境提供科学依据。在返青期设置了放牧(CK)和4个休牧期(20、30、40、50 d)共5个试验处理,采用Biolog-Eco微平板的方法分析了不同返青休牧期下土壤碳代谢的变化,同时测定了土壤性质和土壤微生物生物量碳氮等指标。结果表明:不同返青期休牧下土壤微生物利用碳源能力有CK>30 d>40 d>50 d>20 d的趋势,但差异不显著。对不同种类碳源的利用能力依次为羧酸类>氨基酸类>糖类>酯类>醇类>胺类,且不同返青期休牧处理下微生物对醇类的代谢差异显著(P=0.028),休牧20 d显著高于40 d和50 d。培养第1天在休牧20 d处理下Simpson(D)、Shannon(H)、McIntosh指数(U)都较其他处理高。培养第11天CK处理下,碳代谢功能多样性较高。土壤理化性质及微生物生物量碳氮解释了微生物群落碳代谢的91.1%的变异,其中土壤有机碳解释了8%的变异,且显著影响微生物群落碳代谢(P=0.038)。在试验初期,不同休牧期土壤碳代谢特征差异不显著,且放牧措施有提高碳源利用活性,仅醇类代谢差异显著。土壤有机碳是显著影响微生物群落碳代谢的因素。
the rest-grazing periods in spring can effectively protect the grassland ecological environment.Based on the different restgrazing periods,we can assessment the feasibility of the rest-grazing measures.The response of soil microbial carbolic metabolism to the different rest-grazing periods in spring should be of prime importance.There are complex relationships among soil microbial carbolic metabolism,soil properties and the soil microbes.However,the mechanisms of these effects,particularly in alpine grassland ecosystem,are not clear.We chose the Biolog-Eco plate to determine soil carbon metabolism,and meanwhile measured soil properties,soil microbial biomass carbon and nitrogen in different rest-grazing periods in spring.The soil microbial carbon source utilization capacity in the different rest-grazing periods showed a trend of CK>30 d>40 d>50 d>20 d,but the differences among different treatments were not significant.The utilization ability of six kinds of carbon sources showed a trend of carboxylic acids>amino acids>sugar>esters>alcohols>amine.Microbial metabolism of alcohols was significantly different(P=0.028),to be specific,it was significantly higher in 20 d treatment than in the 50 d treatment.On the first day,the diversities indices of Simpson(D),Shannon(H),McIntosh(U)in the 20d treatment were the higher than the other treatments.However,these indicators in the CK treatments on the eleventh day were higher than the other treatments.Soil physical-chemical properties and microbial biomass carbon(MBC)and biomass nitrogen(MBN)explained 91.1%of the variation in carbon metabolism of the microbial community,among which 8%of the variation was explained by SOC,which significantly affected carbon metabolism of the microbial community(P=0.038).In the early stage of the experiment,there was no significant difference in the characteristics of soil carbon metabolism in different rest-grazing periods,and the grazing measures improved the carbon source utilization activity,and the difference in alcohol metabolism in different treatments was significant.Soil organic carbon significantly affects the carbon metabolism of microbial community.
作者
王晓丽
温军
马玉寿
王彦龙
施建军
周华坤
WANG Xiaoli;WEN Jun;MA Yushou;WANG Yanlong;SHI Jianjun;ZHOU Huakun(Qinghai Academy of Animal and Veterinary Sciences/Qinghai University/State Key Laboratory of Plateau Ecology and Agriculture,Xining 810016,China;Qinghai Key Laboratory of Basin Water Cycle and Ecology/Qinghai Institute of Water Resource and Hydropower Research Co.,Ltd.,Xining 810001,China;Qinghai Provincial Key Laboratory of Restoration Ecology for Cold Regions/Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008 China)
出处
《生态环境学报》
CSCD
北大核心
2020年第5期961-970,共10页
Ecology and Environmental Sciences
基金
国家重点研发计划课题(2016YFC0501904)
国家自然科学基金青年项目(31700454)
青海省自然科学基金项目(2018-ZJ-939Q)
青海省重点研发与转化计划-科技成果转化项目(2018-SF-137)。
关键词
土壤微生物碳代谢
返青期休牧
Biolog-Eco板
微生物墒
土壤微生物生物量碳
soil microbial carbolic metabolism
Rest-grazing in the greenup period
Biolog-Eco plate
soil microbial quotient
soil microbial biomass carbon
