摘要
目的了解红带锥蝽在广东省的地理分布、形态学特征和分子生物学特征。方法选取粤西地区和珠江三角洲地区8个地市共23个县(市、区)开展调查,采取光诱法和人诱法进行搜捕。对捕获的锥蝽进行形态学和分子生物学鉴定,提取锥蝽基因组DNA,PCR扩增细胞色素b(Cytb)基因,测序并进行序列比对。结果调查共捕获红带锥蝽273只,其中若虫36只,成虫237只,分布在广东省的8个地市共23个县(市、区)。经形态学特征和基因序列分析,采集到的锥蝽为红带锥蝽,与广西合浦红带锥蝽Cytb基因(GenBank:MH368018.1)序列同源性最高,为94.91%。调查共收集到9起锥蝽叮咬人的事件报告。结论红带锥蝽广泛分布于广东省粤西地区和珠三角地区,居民被叮咬的事件时有发生,应加强对红带锥蝽的监测和控制。
Objective To describe the geographical distribution of Triatoma rubrofasciata(T.rubrofasciata)in Guangdong Province,and to identify the morphological and molecular biological characteristics of this species.Methods The investigation was carried out in 23 counties of 8 cities in western Guangdong and Pearl River Delta.T.rubrofasciatae were collected by manual inspections and light trapping,and subjected to morphological identification using light microscopy.The DNA of T.rubrofasciata was extracted,and Cytb gene was amplified and sequenced with PCR and aligned with that in the GenBank.Results A total of 273 triatomines,36 of which were nymphae and 237 were adults,were captured from 23 different counties in 8 cities in Guangdong.All collected bugs were identified as T.rubrofasciata by morphology.The phylogenetic tree based on the Cytb gene showed that the sequence of T.rubrofasciata from Guangdong was highly coincident with that of this species from Hepu County of Guangxi Province.The sequence homology was up to 94.91%(GenBank:MH368018.1).In addition,nine incidences of triatomines bite were observed during the investigation.Conclusion T.rubrofasciata is widely distributed in western areas and the Pearl River Delta of Guangdong Province,and the incidence of T.rubrofasciata bite often occur in residents in these areas,which suggests that enhanced surveillance and control of T.rubrofasciata are necessary.
作者
廖宇煌
卢文成
毛强
张启明
何宇驰
邓卓晖
LIAO Yu-huang;LU Wen-cheng;MAO Qiang;ZHANG Qi-ming;HE Yu-chi;DENG Zhuo-hui(Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 511430,China;Zhanjiang Center for Disease Control and Prevention)
出处
《热带病与寄生虫学》
2020年第3期145-150,共6页
Journal of Tropical Diseases and Parasitology
基金
广东省医学科学技术研究基金(B2020140)。
关键词
红带锥蝽
调查
地理分布
形态特征
广东省
Triatoma rubrofasciata
Investigation
Geographical distribution
Morphological characteristic
Guangdong Province
