摘要
目的 探索胶质细胞源性神经营养因子(GDNF)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导帕金森病小鼠步态运动行为的影响.方法 选择C57BL/6小鼠48只,将小鼠随机分成对照组、模型组和GDNF组,对照组仅给予生理盐水,而其余两组注射MPTP造模.对照组和模型组给予等体积生理盐水,GDNF组腹腔注射GDNF.给药7 d后,比较三组小鼠爬杆、悬挂及转棒实验等步态运动行为.结果 模型组小鼠在第一次注射MPTP后10~20 min均出现一定程度的翘尾、震颤等症状,且在持续4~5 h后症状有所减轻,但小鼠仍存在动作迟缓和活动变少等变化;建模完成后模型组和GDNF组小鼠均出现动作迟缓、震颤、步态不稳等体征;对照组小鼠未见上述症状,且注射GDNF溶液后,上述症状有一定程度的缓解.三组小鼠调头时间、爬杆上半部分时间、爬杆下半部分时间和总时间差异均存在统计学意义(P<0.05);对照组小鼠调头时间、爬杆上半部分时间、爬杆下半部分时间和总时间明显低于模型组和GDNF组,且GDNF组小鼠调头时间、爬杆上半部分时间、爬杆下半部分时间和总时间明显低于模型组(P<0.05).三组小鼠悬挂评分和潜伏期差异均存在有统计学意义(P<0.05);对照组小鼠悬挂评分和潜伏期水平明显高于模型组和GDNF组(P<0.05),且GDNF组小鼠悬挂评分和潜伏期水平明显高于模型组(P<0.05).三组小鼠跌落时间差异有统计学意义(P<0.05);对照组跌落时间明显高于模型组和GDNF组,且GDNF组跌落时间明显高于模型组(P<0.05).结论 GDNF应用于帕金森模型小鼠可有效缩短爬杆时间,延长悬挂时间,增加转棒时间,进而改善模型小鼠步态运动行为.
Objective To explore the effect of GDNF on gait motor behavior of mice with Parkinson's disease induced by MPTP.Methods 48 C57BL/6 mice were randomly divided into control group,model group and GDNF group.The control group was only given normal saline,while the other groups were injected with MPTP.The mice in the control group and model group were given the same volume of normal saline.The mice in the GDNF group were given intragastric administration of GDNF.After 7 days of administration,the gait movement behaviors of the three groups,such as pole climbing,suspension and rod turning test were compared.Results After the first injection of MPTP,the mice in the model group showed some symptoms such as tailings and tremors,and the symptoms were reduced after 4 to 5 hours,but the mice still had changes such as slow motion and less activity.There were significant differences in U-turn time,upper half time,lower half time and total time among the three groups(P<0.05),the U-turn time,upper half time,lower half time and total time in the control group were significantly lower than those in the model group and GDNF group(P<0.05),and the U-turn time,upper half time,lower half time and total time in the GDNF group were significantly lower than those in the model group(P<0.05).There were significant differences in suspension score and latency among the three groups(P<0.05),suspension score and latency in the control group were significantly higher than those in the model group and GDNF group(P<0.05),and suspension score and latency time in the GDNF group were significantly higher than those in the model group(P<0.05).There were significant differences in the fall time among the three groups(P<0.05),the fall time in the control group was significantly higher than that of the model group and GDNF group(P<0.05),and the fall time in the GDNF group was significantly higher than that of the model group(P<0.05).Conclusion The application of GDNF in Parkinson's model mice can effectively shorten the rod climbing time,prolong the suspension time,increase the rod rotation time,thus optimizing the gait motor behavior of Parkinson's model mice.
作者
叶盛
石宇
YE Sheng;SHI Yu(Department of Obstetrics and Gynecology,Shenzhen Hospital of Peking University,Shenzhen,Guangdong 518036,China)
出处
《中国处方药》
2020年第8期30-32,共3页
Journal of China Prescription Drug
基金
广东省医学科研基金(B2018090)。
