摘要
采用直接水浴加热双水相法提取巴山雀舌茶树老叶中的黄酮,对分相盐进行了筛选,研究了乙醇体积分数、分相盐的用量、提取时间、提取温度变化对黄酮得率的影响.在单因素实验结果的基础上,以L9(34)正交设计实验得到了黄酮的最优提取条件:乙醇体积分数为70%,硫酸铵用量为5g/30mL,提取温度为60℃,提取时间为70min;此条件下进行验证实验,黄酮得率均值为6.61%,相对标准偏差为0.63%;提取产物对DPPH·清除率与其加入量呈良好的正相关关系,用浓度为0.0002mol/L的DPPH·进行实验,最大清除率为71.18%.双水相体系提取茶树老叶中的黄酮操作简便,对茶叶生产中副产物的综合利用有积极意义.
Dual aqueous phase extraction of flavonoids in old Bashan finch tea leaves was studied.By water-bath heating,effects of phylogenetic salt species,ethanol volume fraction,phylogenetic salt dosage,extraction time,and extraction temperature upon yield of flavonoids,were explored.By L 9(34)orthogonal tests,the optimal extraction conditions were acquired,namely,ethanol volume fraction of 70%,ammonium sulfate dosage of 5 g/30mL,extraction temperature of 60℃,and extraction time of 70min,under which the yield of flavonoids could reach 6.61%.The clearing rate of extract for DPPH·had good positive correlation with the added amount of extract.The clearing ability of extract to DPPH·was better than that of rutin.
作者
阮尚全
张红
窦文超
聂思佳
项婷
刘红
刘润清
RUAN Shangquan;ZHANG Hong;DOU Wenchao;NIE Sijia;XIANG Ting;LIU Hong;LIU Runqing(College of Chemistry and Chemical Engineering,Neijiang Normal University,Neijiang,Sichuan 641100,China;Key Laboratory of Fruit Waste Treatment and Resource Recycling of the Sichuan Provincial College,Neijiang Normal University,Neijiang,Sichuan 641100,China)
出处
《内江师范学院学报》
2020年第8期56-60,65,共6页
Journal of Neijiang Normal University
基金
国家级大学生创新创业训练计划项目(X2018027)
内江师范学院大学生自然科学基金项目(18ZA0004)
四川省教育厅自然科学基金重点项目(15ZA0291)。
关键词
茶树叶
双水相体系
水浴法
黄酮
自由基
tea leaves
dual aqueous phase system
water-bath method
flavonoids
antioxidant activity
