摘要
利用5个DNA片段及12个微卫星标记(SSR)对姜状三七(Panax zingiberensis C.Y.Wu et K.W.Feng)的遗传多样性和遗传分化进行分析。结果显示,与其他人参属(Panax)植物相比:姜状三七具有相对较高物种水平的核苷酸多态性和等位基因数;在居群水平上,景谷居群具有最高的核苷酸多态性和等位基因数,而江城居群最低。AMOVA分析结果表明,姜状三七在物种水平上具有一定程度的遗传分化,但不显著;在居群水平,江城居群与其他居群间的分化程度最高,其他居群间遗传分化不明显。Structure分析结果也显示江城居群与其他居群被聚类到不同分组,且形态特征相近的样本并未聚到一起,同一分布点的样本遗传成分更相似。
Panax zingiberensis C.Y.Wu et K.W.Feng is a perennial herbaceous plant belonging to the genus Panax(Araliaceae).It is an endangered species mainly distributed in southeastern and southern Yunnan,China.Like other species of Panax,P.zingiberensis(otherwise known as“Yesanqi”)has high medicinal value and is used as a substitute of P.notoginseng by local people.In this study,the genetic diversity and genetic differentiation of P.zingiberensis was analyzed using data obtained from five DNA fragments and 12 microsatellite(SSR)markers.Results showed that the total nucleotide diversity of P.zingiberensis was just 0.00068 at the species level based on the combined analyses of four chloroplast fragments(psbA-trnH,psbM-trnD,rps16,and trnL-trnF),whereas the nucleotide diversity of nuclear ITS(Pi=0.01011)was high.In the 12 polymorphic microsatellite loci,a total of 89 alleles were identified,and the average number of alleles per locus was 7.417.Compared to other Panax species,P.zingiberensis had a relatively high level of nucleotide diversity and number of alleles.At the population level,population JG had the highest nucleotide diversity and number of alleles,whereas population JC had the lowest.AMOVA indicated that genetic variation among populations(55.03%)in the cpDNA fragment was greater than that within populations(44.97%).However,for the ITS fragment and SSR locus,genetic variation among populations(30.23%and 35.90%,respectively)was much lower than that within populations(69.77%and 64.10%,respectively).This indicated that P.zingiberensis had a certain degree of genetic differentiation at the species level,but it was not significant(P=0.01 or 0.05).At the population level,based on genetic differentiation indices(Fst′,Gst′(Nei),and Rho)inferred from SSR data,population JC exhibited high differentiation from the other four populations,whereas no differentiation was observed among these populations.Structure analysis showed that JC and other populations were clustered into different genetic groups,respectively.Combined with phenotypic characteristics of the samples,structure analysis also showed that those individuals with similar morphological characteristics were not clustered together,whereas individuals of the same population had more similar genetic components.This study should help in the conservation of wild resources of P.zingiberensis.
作者
吴丽新
龚洵
潘跃芝
Wu Li-Xin;Gong Xun;Pan Yue-Zhi(Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;University of Chinese Academy of Sciences, Beijing 100049, China)
出处
《植物科学学报》
CAS
CSCD
北大核心
2020年第4期525-535,共11页
Plant Science Journal
基金
国家自然科学基金项目(31570339)。
关键词
姜状三七
遗传多样性
遗传分化
SSR标记
DNA测序
Panax zingiberensis
Genetic diversity
Genetic differentiation
SSR markers
DNA sequencing
