摘要
目的分析黄褐斑动物模型应用现状,为探讨黄褐斑理论知识、探究新疗法、研制新药物打好动物实验基础,提高治疗黄褐斑的科学性、严谨性、安全性。方法以"黄褐斑"为主题,检索中国知网、万方数据库、维普数据库中关于黄褐斑的实验研究文献,时间范围分别为"1960-2019、1990-2019、1979-2019"。归纳分析文献中动物模型的应用、相关指标的检测等。结果共筛选出104篇实验研究文献,以采用雌性KM小鼠复制黄褐斑动物模型最为常见,其造模方法以紫外线照射最为常用;造模时间以30 d最为常见,最短不少于7 d;给药时间大多分布在28~31 d。在指标的测量中,可分为表观指标、生化指标及病理指标,以生化指标的检测为主,侧重于皮肤及血清中氧化相关因子的变化。结论目前对黄褐斑的实验研究及理论探讨与黄褐斑发病现状严重失衡,应增加动物实验研究;建立复合动物模型、具有中医病症特点的动物模型是未来重要发展方向。此外,对药物作用机制的研究仅局限于体内、体表氧化与抗氧化失衡,忽略内分泌失调等相关因素,随着体表"神经-内分泌-免疫"(NEI)网络的提出与深入研究,探讨在黄褐斑的治疗中,是否可以通过调节体内、体表NEI网络而发挥整体、局部疗效可能是未来重要研究方向,值得进一步深入探讨。
Objective To analyze the use of animal models of chloasma,and to lay a good foundation for future animal studies designed to explore the basic biology of chloasma,test new therapies,and develop new drugs,so as to generate scientific,rigorous,and safe treatment approach for chloasma.Methods The CNKI,Wanfang and Weipu databases were searched for all publication pertaining to the key word“chloasma”,with the following time ranges:1960–2019,1990–2019,and 1979–2019.The application of animal models in the literature and the detection of relevant indicators were analyzed and summarized.Results A total of 104 experimental studies were retrieved.Female KunMing(KM)mouse models of chloasma were the most common,and UV irradiation was the most common method used to induce chloasma.The most common experimental duration was 30 days,and all of the studies lasted for at least 7 days.Drugs were typically administered for between 28 and 31 days.The indicators that were assessed can be divided into apparent indicators,biochemical indicators,and pathological indicators.Assessment of biochemical indicators was most common,primarily focusing on changes in oxidation-related factors in the skin and serum.Conclusions There is a serious imbalance between experimental research and theoretical discussions related to chloasma and the clinical need for chloasma treatments.The establishment of a compound animal model and an animal model with TCM disease characteristics will be an important focus for future research.Existing studies of the mechanisms of drug activity are limited to imbalances in the body,surface oxidation,and anti-oxidation,and ignore factors related to endocrine disorders.In-depth studies of body surface NEI networks to identify factors that can alter body properties may be an important research direction for developing new treatments for chloasma,whether they exhibit full or partial effectiveness.
作者
武晏屹
田硕
苗明三
WU Yanyi;TIAN Shuo;MIAO Mingsan(Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第8期70-75,共6页
Chinese Journal of Comparative Medicine
基金
国家中医药管理局中医药标准化专项(2017-149-11)
国家国际合作基地(2016-65)
河南中医药大学创新创业团队(2019CXCY003)。
