摘要
背景:作者所在课题组前期研究发现,巴戟天醇提物能有效改善卵巢切除肥胖大鼠的骨质量和体质量,但具体机制尚不明确。该研究以瘦素及瘦素受体为切入点,探讨巴戟天醇提物对卵巢切除肥胖模型大鼠脂代谢及骨代谢的影响。目的:研究巴戟天醇提物对卵巢切除肥胖大鼠脂代谢及骨代谢的影响。方法:将雌性SD大鼠160只随机分为骨质疏松组120只、假手术组40只。骨质疏松组通过切除双侧卵巢建立绝经后骨质疏松症模型,术后骨质疏松组再随机分为:卵巢切除+正常饮食组、卵巢切除+高脂饮食组以及卵巢切除+高脂饮食+巴戟天醇提物组,各40只。假手术组、卵巢切除+正常饮食组用普通饲料喂养,卵巢切除+高脂饮食组、卵巢切除+高脂饮食+巴戟天醇提物组用高脂饲料喂养。高脂巴戟天醇提物组用20 g/kg巴戟天醇提物灌胃,1次/d,余下3组用2 mL生理盐水灌胃。实验于2018年9月经厦门大学附属福州第二医院动物实验伦理委员会批准,审批号:20180019。结果与结论:与假手术组相比,卵巢切除大鼠血清中瘦素、瘦素受体、骨保护素以及高密度脂蛋白胆固醇浓度较低(P<0.05),而骨钙素、RANKL、抗酒石酸酸性磷酸酶5b、胆固醇、三酰甘油和低密度脂蛋白胆固醇的浓度较高(P<0.05)。与卵巢切除+高脂饮食组相比,卵巢切除+高脂饮食+巴戟天醇提物组大鼠血清瘦素、瘦素受体、骨保护素和高密度脂蛋白胆固醇均有所提高(P<0.05),而骨钙素、RANKL、抗酒石酸酸性磷酸酶5b、胆固醇、三酰甘油和低密度脂蛋白胆固醇出现不同程度下降(P<0.05)。提示巴戟天醇提物能够上调卵巢切除肥胖大鼠血清中瘦素及瘦素受体的表达,进而改善卵巢切除肥胖大鼠的骨代谢及脂代谢水平异常。
BACKGROUND:Previous studies have showed that the alcohol extract of Morinda officinalis can effectively improve the bone quality and body mass of obese rats after ovariectomy.However,the exact mechanism is unclear.In this study,leptin and leptin receptor were used as the breakthrough point to investigate the effect of alcohol extract of Morinda officinalis on lipid metabolism and bone metabolism in ovariectomized obese rats.OBJECTIVE:To investigate the effects of alcohol extract of Morinda officinalis lipid metabolism and bone metabolism in ovariectomized obese rats.METHODS:A total of 160 SPF female Sprague-Dawley rats were randomly divided into an osteoporosis group(n=120)and a sham operation group(n=40).A postmenopausal osteoporosis model was made in the osteoporosis group by removing both ovaries.After modeling,rats in the osteoporosis group were randomly subdivided into a normal diet group,a high-fat diet group and a high-fat diet+Morinda officinalis alcohol extract group,with 40 rats in each group.The sham operation group and the normal diet group were fed with ordinary diet,while the high-fat diet group and the high-fat diet+Morinda officinalis alcohol extract group were fed with high-fat diet.The high-fat diet+Morinda officinalis alcohol extract group was gavaged with 20 g/kg Morinda officinalis alcohol extract once a day,and the remaining three groups were gavaged with 2 mL of normal saline.The study protocol was approved by the Animal Ethic Committee of Fuzhou Second Hospital of Xiamen University in September 2018 with an approval No.20180019.RESULTS AND CONCLUSION:Compared with the sham operation group,serum leptin,leptin receptor,osteoprotegerin and high-density lipoprotein cholesterol levels were significantly lower in ovariectomized rats(P<0.05),whereas osteocalcin,RANKL,tartrate-resistant acid phosphatase 5b,cholesterol,triacylglycerol,and low-density lipoprotein cholesterol levels were significantly higher in ovariectomized rats(P<0.05).Compared with the high-fat diet group,serum leptin,leptin receptor,osteoprotegerin and high-density lipoprotein cholesterol levels were significantly increased in the high-fat diet+Morinda officinalis alcohol extract group(P<0.05),whereas osteocalcin,RANKL,tartrate-resistant acid phosphatase 5b,cholesterol,triacylglycerol,and low-density lipoprotein cholesterol levels were decreased to different extents in the high-fat diet+Morinda officinalis alcohol extract group(P<0.05).To conclusion,the alcohol extract of Morindus officinalis can up-regulate the leptin and leptin receptor expression in serum of ovariectomized obese rats,so as to improve the abnormal bone metabolism and lipid metabolism in ovariectomized obese rats.
作者
黄朱宋
林煜
陈翔
蓝锦福
关勇
高曦
Huang Zhusong;Lin Yu;Chen Xiang;Lan Jinfu;Guan Yong;Gao Xi(Department of Orthopaedics,Fuzhou Second Hospital of Xiamen University,Fuzhou 350007,Fujian Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第2期205-210,共6页
Chinese Journal of Tissue Engineering Research
基金
福州市卫生计生中青年科学研究项目(2018-S-wq17),项目负责人:高曦。
关键词
巴戟天醇提物
卵巢切除大鼠
肥胖
瘦素
瘦素受体
脂代谢
骨代谢
绝经后骨质疏松
alcohol extract of Morindus officinalis
ovariectomized rats
obesity
leptin
leptin receptor
lipid metabolism
bone metabolism
postmenopausal osteoporosis
