摘要
目的观察微管相关蛋白Tau(MAPT)在肾透明细胞癌(ccRCC)中的表达情况,与临床病理参数的关系以及对预后的评估价值。方法分析癌症基因组图谱(TCGA)和本院验证队列中ccRCC肿瘤组织和癌旁正常组织中MAPT的表达情况,分析与临床病理参数的关系以及生存情况。在786-O和Caki-1细胞系中敲降MAPT,观察MAPT对生物学功能的影响。结果在TCGA队列中,MAPT的表达与病理T分期(pT)、AJCC分期、Fuhrman分级和是否存在远处转移显著相关(P<0.05);在验证队列中,MAPT的表达与T分期(pT)和AJCC分期显著相关(P<0.05)。MAPT高表达组的总体生存率显著高于MAPT低表达组(P<0.05)。敲降MAPT可能促进ccRCC细胞的增殖和侵袭。结论ccRCC中MAPT低表达可能与预后不良有关。敲降MAPT可能促进ccRCC细胞的增殖和侵袭。
Objective To observe the expression of microtubule associated protein Tau(MAPT)in clear cell renal cell carcinoma(ccRCC),its relationship with clinicopathological parameters and its prognostic value.Methods To analyze the expression of MAPT in ccRCC tumor tissue and adjacent normal tissue,the relationship between MAPT and clinicopathological parameters,as well as the survival status.MAPT was knocked down in 786-O and Caki-1 cell lines to observe the effect of MAPT on biological function.Results In TCGA cohort,MAPT expression was significantly correlated with pathological T stage(pT),AJCC stage,Fuhrman stage and distant metastasis(P<0.05);in validation cohort,MAPT expression was significantly correlated with T stage(pT)and AJCC stage(P<0.05).The overall survival rate of MAPT high expression group was significantly higher than that of MAPT low expression group(P<0.05).Knockdown of MAPT may promote the proliferation and invasion of ccRCC cells.Conclusion Low MAPT expression in ccRCC may be related to poor prognosis.Knockdown of MAPT may promote the proliferation and invasion of ccRCC cells.
作者
赵凌昆
谭九峰
崔万里
晋学飞
ZHAO Ling-kun;JIN Xue-fei;TAN Jiu-feng(School of Clinical Medicine,Jiamusi University,Jiamusi 154007,China)
出处
《中国实验诊断学》
2020年第8期1347-1351,共5页
Chinese Journal of Laboratory Diagnosis
