摘要
目的研究脉冲电磁场(PEMF)对去卵巢骨质疏松(OVX-OP)大鼠的作用及机制。方法将6月龄雌性SD大鼠随机分为假手术组(S组)、模型组(M组,双侧卵巢切除)、雌激素治疗组(E组,M组基础上进行雌激素治疗)和PEMF组(M组基础上进行PEMF治疗),每组各10只,术后饲养8周后进行治疗,治疗持续8周。称取治疗前后各组大鼠体重;采用双能X射线实验动物骨密度测定仪检测各组大鼠右肱骨骨密度。治疗结束后,采用半自动图像数字化分析仪对胫骨上1/3作静态骨形态计量学检测;采用免疫印迹(WB)检测各组大鼠骨组织Smad泛素化调节因子1(Smurf1)蛋白表达量。结果与S组比较,M组、E组、PEMF组精神状况欠佳,饮食、活动及大小便未见异常。治疗前,与S组比较,M组、E组、PEMF组大鼠体重显著增加(P<0.05),骨密度值显著降低(P<0.05)。治疗后,E组、PEMF组大鼠体重显著降低(P<0.05),骨密度值较治疗前显著增加(P<0.05);与S组比较,M组大鼠骨密度值、BV/TV、Tb.N、Tb.Th显著降低(P<0.05),体重、Tb.Sp、Smurf1蛋白水平显著增加(P<0.05);与M组比较,E组、PEMF组大鼠骨密度值、BV/TV、Tb.N、Tb.Th显著升高(P<0.05),体重、Tb.Sp、Smurf1蛋白水平显著降低(P<0.05);E组与PEMF组比较,大鼠各指标差异无统计学意义(P>0.05)。结论PEMF可增加骨密度、改善骨形态变化,发挥抗骨质疏松的作用,可能与Smurf 1蛋白表达下调有关。
Objective To study the effect and mechanism of pulsed electromagnetic field(PEMF)on ovariectomized osteoporosis(OVX-OP)rats.Methods Six-month-old female SD rats were randomly divided into four groups:sham operated group(S group),model group(M group,bilateral ovariectomy),estrogen treatment group(E group,estrogen treatment based on M group)and PEMF group(PEMF treatment based on M group),with 10 rats in each group,and they were treated for 8 weeks after 8 weeks of feeding.The weight of rats in each group before and after treatment was weighed;the bone mineral density of right humerus was measured by dual energy X-ray experimental animal bone densitometer.At the end of treatment,the static bone morphometry of 1/3 upper tibia was measured by the semi-automatic digital image analyzer;and Western blotting(WB)was used to detect the protein expression of Smad ubiquitin regulatory factor 1(Smurf1)in bone tissue of rats in each group.ResultsCompared with S group,the mental state of M group,E group and PEMF group was not good,and there was no abnormality in diet,activity and defecation.Before treatment,compared with S group,the weight of rats in M group,E group and PEMF group increased significantly(P<0.05),while the bone mineral density decreased significantly(P<0.05);while after treatment,the weight of rats in E group and PEMF group decreased significantly(P<0.05),and the bone mineral density increased significantly(P<0.05);compared with S group,the bone mineral density,BV/TV,Tb.N,and Tb.Th in M group decreased significantly(P<0.05),while the weight of rats,Tb.Sp and the protein level of Smurf1 increased significantly(P<0.05);compared with group M,the bone mineral density,BV/TV,Tb.N and Tb.Th in E group and PEMF group increased significantly(P<0.05),while the weight of rats,Tb.Sp and the protein level of Smurf1 decreased significantly(P<0.05);There was no significant difference between E group and PEMF group(P>0.05).Conclusion PEMF may improve bone morphology by up-regulating the expression of Smurf1 protein,and then play the role of anti osteoporosis,which may be related to the down-regulation of Smurf1 protein expression.
作者
孙智路
尹剑
黄开亮
谭位华
刘静
SUN Zhilu;YIN Jian;HUANG Kailiang;TAN Weihua;LIU Jing(Department of Emergency,The First Affiliated Hospital of Nanhua University,Hengyang 421001;Department of Rehabilitation Medicine,Hengyang 421001,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2020年第8期1093-1097,共5页
Chinese Journal of Osteoporosis
基金
国家自然科学基金(81674045)
湖南省卫健委课题(20200410)
湖南省自然科学基金(2019JJ50544)。
