摘要
目的探讨胃癌的临床病理特征与鼠类肉瘤病毒癌基因(kirsten rat sarcoma viral oncogene,KRAS)突变的相关性。方法选择120例胃癌患者(胃癌组)与120例胃良性病变患者(良性组),提取2组患者病理组织的DNA,对KRAS基因第2号外显子进行扩增,记录KRAS基因突变情况并进行相关性分析。结果胃癌组的KRAS基因突变率为75.0%,显著高于良性组的15.0%(P<0.05)。在120例胃癌患者中,不同临床分期、淋巴结转移、分化程度、远处转移、浸润深度患者的KRAS基因突变率对比,差异有统计学意义(P<0.05)。胃癌组患者随访至今,平均随访时间为(15.22±2.48)月,死亡12例,死亡率为10.0%。单因素和多因素Cox回归分析显示KRAS基因突变率、临床分期、淋巴结转移、分化程度、远处转移、浸润深度都为影响胃癌患者预后的危险因素(P<0.05)。结论KRAS基因突变在胃癌组织中比较常见,与患者的临床病理特征显著相关,可以作为预测胃癌发生和预后的潜在生物标志物。
Objective To investigate the relationship between the clinicopathological features of gastric cancer and the gene mutation of sarsten rat sarcoma viral oncogene(KRAS).Methods 120 patients with gastric cancer(gastric cancer group)and 120 patients with benign gastric lesions(benign group)were enrolled.The DNA of pathological tissues of the 2 groups were extracted,and the KRAS gene mutation in the second exon were amplified by the PCR.Results The KRAS gene mutation rate in the gastric cancer group were 75.0%,which were significantly higher than that in the benign group(15.0%)(P<0.05).In 120 patients with gastric cancer,the KRAS gene mutation rates in patients with different clinical stages,lymph node metastasis,differentiation,distant metastasis,and invasive depth were statistically significant(P<0.05).The patients in the gastric cancer group were followed up for an average of 15.22±2.48 months,and there were 12 patients were died,and the mortality rates were 10.0%.Univariate and multivariate Cox regression analysis showed that KRAS gene mutation rate,clinical stage,lymph node metastasis,differentiation,distant metastasis,and depth of invasion were all risk factors for the prognosis of patients with gastric cancer(P<0.05).Conclusion KRAS gene mutation is more common in gastric cancer tissues and is significantly correlated with the clinicopathological features of patients.It could be used as potential biomarker for predicting the occurrence and prognosis of gastric cancer.
作者
姚书鹏
李军
张友满
YAO Shupeng;LI Jun;ZHANG Youman(Ankang Central Hospital,Ankang,725000)
出处
《实用癌症杂志》
2020年第8期1249-1252,共4页
The Practical Journal of Cancer
基金
陕西省自然科学基础研究计划项目(编号:S2015YFJM2049)。
关键词
鼠类肉瘤病毒癌基因
基因突变
胃癌
临床病理特征
Kirsten rat sarcoma viral oncogene
Gene mutation
Gastric cancer
Clinicopathological features
