摘要
背景:Th17细胞的特异性细胞因子白细胞介素17在退变的腰椎间盘组织中含量显著升高,白细胞介素21对Th17细胞的分化与增殖起到重要的作用。目的:探讨白细胞介素21在腰椎间盘退变中的作用与意义。方法:取40份髓核组织标本,其中32份取自腰椎间盘退变患者,8份取自腰椎骨折患者,同时取两组患者外周血样本。所有患者均收治于南通大学附属医院脊柱外科并经MRI及临床查体确诊。采用免疫组化法检测两组髓核组织中白细胞介素21的表达,ELISA检测两组髓核组织和外周血中白细胞介素21、白细胞介素17和白细胞介素6水平,并进行Pearson相关分析。结果与结论:免疫组化结果发现试验组髓核组织中白细胞介素21阳性率明显高于对照组(P<0.01)。ELISA检测结果发现试验组髓核组织及外周血中白细胞介素21、白细胞介素17和白细胞介素6水平均明显高于对照组(P<0.01)。试验组髓核组织中白细胞介素21水平与其相关因子白细胞介素17、白细胞介素6水平呈正相关。结果表明白细胞介素21参与了腰椎间盘退变时发生的炎症反应和自身免疫反应。
BACKGROUND:Interleukin-17,as the specific marker of Th17 cells,is highly expressed in degenerative lumbar disc tissue.Interleukin-21 plays an important role in proliferation and differentiation of Th17 cells.OBJECTIVE:To test the expression of interleukin-21 in nucleus pulpous tissue and peripheral blood of patients who accepted the surgery of intervertebral disc degeneration and to analyze the relevant significance.METHODS:In total,40 samples of nucleus pulpous tissues were obtained,32 from intervertebral disc degeneration(IDD)and 8 from lumbar fracture(control group).Peripheral blood samples were collected from the same cohort.All patients were confirmed by physical examination and MRI and then admitted to the Spine Centre of Affiliated Hospital of Nantong University.We detected the positive signal of interleukin-21 in nucleus pulpous tissue through immunohistochemical method,and measured the levels of interleukin-21,interleukin-17 and interleukin-6 in the nucleus pulpous and peripheral blood by ELISA.Pearson’s correlation analysis was performed thereafter.RESULTS AND CONCLUSION:There were more positive signals of interleukin-21 in the IDD group than the control group.The expression levels of interleukin-21,interleukin-17 and interleukin-6 were significantly higher in the IDD group than the control group as detected by ELISA.The level of interleukin-21 was positively correlated with the expression of interleukin-17 and interleukin-6.These findings indicate that interleukin-21 is involved in inflammatory and autoimmune responses during the development of IDD.
作者
杨洋
姚羽
沈孝天
刘佳佳
薛建华
Yang Yang;Yao Yu;Shen Xiaotian;Liu Jiajia;Xue Jianhua(Department of Trauma Center,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China;Department of Orthopedics,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China;Nantong University,Nantong 226001,Jiangsu Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第5期690-694,共5页
Chinese Journal of Tissue Engineering Research
基金
南通市科技项目(MS12018057),项目负责人:杨洋。
关键词
腰椎
椎间盘
退变
髓核
白细胞介素21
TH17细胞
细胞因子
炎症
lumbar disc
intervertebral disc
degeneration
nucleus pulposus
interleukin-21
Th17 cell
cytokine
inflammation
