摘要
目的:探究d-δ-生育酚对人晶状体上皮SRA细胞生长的影响以及相关分子机制,为d-δ-生育酚用于治疗与预防后发性白内障提供实验依据。方法:实验分为6组,即空白对照组及实验组,即5个不同浓度的d-δ-生育酚(40、60、80、100、120μmol/L),噻唑兰(MTT)法检测各组细胞的增殖抑制率。在倒置显微镜下观察人晶状体上皮SRA细胞形态。流式细胞仪检测细胞周期,蛋白印迹法(Western blot)检测bcl-2、bax、Cyclin D1、P21蛋白表达。结果:随着d-δ-生育酚的浓度逐渐增加,SRA细胞较对照组细胞明显减少;作用时间的延长,SRA细胞增殖抑制率逐渐增高(P<0.05);S期细胞所占比例与对照组比较逐渐增高,细胞被阻滞于S期(P<0.05);d-δ-生育酚干预人晶状体上皮SRA细胞48h后,人晶状体上皮SRA细胞P21、Cyclin D1和bcl-2表达量逐渐降低,bax表达量逐渐增高(P<0.01)。结论:d-δ-生育酚能明显抑制人晶状体上皮SRA细胞的增殖,阻滞细胞周期于S期。其机制可能是通过抑制bcl-2、P21、Cyclin D1的表达,诱导bax的表达实现的。
AIM:To investigate the effect of d-δ-tocopherol on the growth of human lens epithelial SRA cells and its related molecular mechanism,and to provide experimental basis for the treatment and prevention of posterior cataract with d-δ-tocopherol.METHODS:The experiment was divided into 6 groups,blank control group and experimental group,that is,five different concentrations of d-δ-tocopherol(40,60,80,100,120)μmoL/L.The proliferation inhibition rate of each group was detected by thiazolam(MTT)assay.The morphology of human lens epithelial SRA cells was observed under inverted microscope.Cell cycle was detected by flow cytometry and the expression of bcl-2,bax,Cyclin D1,P21 protein was detected by Western Blot(WB).RESULTS:With the increase of d-δ-tocopherol concentration,the SRA cells decreased significantly compared with the control group;the MTT results showed that with the increase of d-δ-tocopherol concentration,the inhibition rate of cell proliferation increased gradually,the difference was statistically significant(P<0.05);cell cycle:with the increase of the concentration of tocopherol drugs in the experimental group,the proportion of cells in the S phase increased gradually compared with the control group,the cells were blocked in the S phase,the difference was statistically significant(P<0.05);Western blotting:after 48h of d-δ-tocopherol intervention human lens epithelial SRA cells,the P21,Cyclin D1 and bcl-2 expression of human lens epithelial cells gradually decreased,and the expression of bax gradually increased,which was statistically significant(P<0.01).CONCLUSION:d-δ-tocopherol can significantly inhibit the proliferation of human lens epithelial SRA cells and block the cell cycle in S phase.d-δ-tocopherol can inhibit the proliferation of human lens epithelial cells.The proliferation of human lens epithelial SRA cells may be achieved by inhibiting the expression of bcl-2,P21,Cyclin D1 and inducing the expression of bax.
作者
秦程
曾新生
彭勃
唐玉妮
周友弟
宋博
Cheng Qin;Xin-Sheng Zeng;Bo Peng;Yu-Ni Tang;You-Di Zhou;Bo Song(Department of Ophthamology,the Second Affiliated Hospital of Guilin Medical University,Guilin 541199,Guangxi Zhuang Autonomous Region,China;Department of Ophthamology,Affiliated Hospital of Guilin Medical University,Guilin 541100,Guangxi Zhuang Autonomous Region,China)
出处
《国际眼科杂志》
CAS
北大核心
2020年第9期1493-1497,共5页
International Eye Science
基金
广西壮族自治区自筹课题资助(No.Z20190526)。
