摘要
【目的】探讨异翅亚目(Heteroptera)昆虫不同类群间臭腺分泌物化学组分的差异,为该类昆虫寻找新的分类特征并为各类群间的相互关系提供新的证据。【方法】我们采用固相微萃取(solid phase microextraction,SPME)偶联气相色谱-质谱仪(GC-MS)对采自中国的异翅亚目8个总科32个种的臭腺分泌物进行了测定分析;采用典型判别分析法、非参数多元方差分析法、典型变量分析法进行了高级阶元分泌物的化学分类学分析。【结果】典型判别分析结果表明,异翅亚目,尤其是蝽次目中不同总科间的臭腺分泌物具显著差异性,可作为总科间的分类特征;非参数多元方差分析结果支持臭腺分泌物在总科间的显著差异性;结合典型判别分析结果和典型变量分析的结果找到划分异翅亚目8个总科32个种的主要特征性化合物有6类共30种,包括酸类[己酸(hexanoic acid)、丁酸(butanoic acid)、2-己烯酸(2-hexenoic acid)],醇类{2-丁基-1-辛醇(2-butyl-octan-1-ol)、2-己基-1-辛醇(2-hexyl-octan-1-ol)、2-己炔-1-醇(2-hexyn-1-ol)、3,7-二甲基-2-辛烯-1-醇(3,7-dimethyl-2-octen-1-ol)、4,8-二甲基-1-壬醇(4,8-dimethyl-1-nonanol)、2-癸烯-1-醇(2-decen-1-ol)、1-己醇(1-hexanol)、高蒎醇(cis-pinene hydrate)、2-茨醇(borneol)、冰片(2-bornanol)、丙二醇甲醚(1-methoxy-propan-2-ol)、2-乙基1-己醇(2-ethyl-hexan-1-ol)、(6 Z,9Z)-十五烷-1-醇[(6 Z,9Z)-pentadecadien-1-ol]、(E)-9-十六碳烯-1-醇[(E)-9-hexadecen-1-ol]、(S)-3-乙基-4-甲基-1-戊醇[(S)-3-ethyl-4-methyl-pentan-1-ol]、异葑醇(isofenchol)、斯巴醇(spathulenol)},醛类{(E)-2-辛烯醛[(E)-2-octenal]、十二醛(dodecanal)、(Z)-3-己烯醛[(Z)-3-hexenal]、(E)-2-癸烯醛[(E)-2-decenal]、(E,E)-2,4-癸二烯醛[(E,E)-2,4-decadienal]},烷类[2-甲基己烷(2-methyl-hexane)、2,21-二甲基二十二烷(2,21-dimethyl-docosane)],环类[糠醛(furfural)]和萜类[二氢香芹醇(neodihydrocarveol)、二氢松油醇(dihydroterpineol)]。【结论】本研究从异翅亚目8个总科32个物种臭腺分泌物中鉴定的30种特征性化合物在一定程度上可作为异翅亚目总科级阶元的分类特征并为其相互关系提供依据。
【Aim】Exploring the differences in chemical constituents of scent gland secretions among different groups in suborder Heteroptera contributes to finding out new characteristics for these insects and providing new evidence for the relationship among different taxa.【Methods】We analyzed the chemical profiles of scent gland secretions from 32 species of eight superfamilies of Heteroptera collected from China using solid phase microextraction(SPME)coupled with GC-MS.The chemotaxonomy of the secretions was analyzed by canonical discriminant analysis,nonparametric multivariate analysis of variance and canonical variate analysis at the superfamily level.【Results】The results of canonical discriminant analysis show that the compounds secreted by the scent glands exhibit significant variances among different superfamilies in Heteroptera and could be used as taxonomic characteristics for superfamilies in Heteroptera,especially in infraorder of Pentatomomorpha.The results of nonparametric multivariate analysis of variance also support significant variances among superfamilies.The combined results of canonical discriminant analysis and canonical variate analysis show that 30 major characteristic compounds in six groups are found in these insects,including acids(hexanoic acid,butanoic acid,and 2-hexenoic acid),alcohols[2-butyl-octan-1-ol,2-hexyl-octan-1-ol,2-hexyn-1-ol,3,7-dimethyl-2-octen-1-ol,4,8-dimethyl-1-nonanol,2-decen-1-ol,1-hexanol,cis-pinene hydrate,borneol,2-bornanol,1-methoxy-propan-2-ol,2-ethyl-hexan-1-ol,(6 Z,9Z)-pentadecadien-1-ol,(E)-hexadecen-1-ol,(S)-3-ethyl-4-methyl-pentan-1-ol,isofenchol,and spathulenol],aldehydes[(E)-2-octenal,dodecanal,(Z)-3-hexenal,(E)-2-decenal,and(E,E)-2,4-decadienal],alkanes(2-methyl-hexane and 2,21-dimethyl-docosane),cyclics(furfural)and terpenes(neodihydrocarveol and dihydroterpineol).【Conclusion】The 30 characteristic compounds tentatively recognized from the scent secretions of the 32 species of eight superfamilies in Heteroptera can be used as the taxonomic characteristics of superfamilies and the evidences of relationship at the superfamily level.
作者
张嫣
夏炎
陈企发
卜文俊
ZHANG Yan;XIA Yan;CHEN Qi-Fa;BU Wen-Jun(Institute of Entomology,College of Life Sciences,Nankai University,Tianjin 300071,China;Tianjin Key Laboratory of Biosensing and Molecular Recognition,Central Laboratory of Nankai University,College of Chemistry,Nankai University,Tianjin 300071,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2020年第7期870-888,共19页
Acta Entomologica Sinica
基金
国家自然科学基金项目(31820103013)。
